OtherBASIC SCIENCE INVESTIGATIONS

Prolonged High-Fat Feeding Enhances Aortic 18F-FDG and 99mTc-Annexin A5 Uptake in Apolipoprotein E-Deficient and Wild-Type C57BL/6J Mice

Yan Zhao, Yuji Kuge, Songji Zhao, H. William Strauss, Francis G. Blankenberg and Nagara Tamaki
Journal of Nuclear Medicine October 2008, 49 (10) 1707-1714; DOI: https://doi.org/10.2967/jnumed.108.051847

Article Figures & Data

Figures

  • FIGURE 1. 
    FIGURE 1. 

    (Top) Examples of longitudinally incised aortas obtained from ChD–apoE−/−, ChD–wild-type, and normal diet–wild-type mice and stained with Oil Red O (at age of 25 wk). Regions stained red with Oil Red O represent atherosclerotic lesions. (Bottom) Aortic tissues were also examined by cryostat sectioning at same age, and atherosclerotic plaques were confirmed histologically in ChD–apoE−/− mice but not in ChD–wild-type or normal diet–wild-type mice. Bar = 100 μm.

  • FIGURE 2. 
    FIGURE 2. 

    Percentages of surface area positively stained with Oil Red O in descending aorta of apoE−/− mice after different periods of high-fat feeding (ChD–apoE−/− mice). Percentages of positively stained surface area significantly increased with age (F = 53.38; P < 0.001). No significant differences were observed in percentages of positively stained area between groups given 18F-FDG and 99mTc-annexin A5 (F = 0.51; P = 0.48).

  • FIGURE 3. 
    FIGURE 3. 

    Correlations between percentages of aortic surface area occupied by atherosclerotic plaques and aortic tracer uptake in ChD–apoE−/− mice. Correlations were calculated with linear regression analysis for groups given 18F-FDG (A) and 99mTc-annexin A5 (B).

Tables

  • TABLE 1

    Body Weight and Serum Lipid Levels

    Mean ± SD in mice that were:
    ParameterMouse group10 wk old18 wk old25 wk old
    Body weight (g)Normal diet–wild-type21.34 ± 0.6726.19 ± 1.77*28.58 ± 1.58*†
    ChD–wild-type26.92 ± 1.23‡36.48 ± 3.13*‡40.39 ± 3.28*†‡
    ChD–apoE−/−28.76 ± 2.02‡§41.02 ± 6.57*‡46.04 ± 5.35*‡§
    Total cholesterol level (mg/dL)Normal diet–wild-type73.42 ± 5.5868.50 ± 6.3678.42 ± 11.84
    ChD–wild-type143.00 ± 17.77‡157.77 ± 18.51‡179.38 ± 27.10*†‡
    ChD–apoE−/−1,386.17 ± 330.98‡§2,097.00 ± 539.89*‡§1,716.00 ± 383.81*†‡§
    LDL cholesterol level (mg/dL)Normal diet–wild-type15.10 ± 5.5312.52 ± 5.2019.32 ± 4.31†
    ChD–wild-type44.32 ± 12.33‡59.34 ± 12.26*‡73.65 ± 18.93*†‡
    ChD–apoE−/−1,358.22 ± 337.42‡§2,056.33 ± 535.88*‡§1,677.95 ± 375.12*†‡§
    HDL cholesterol level (mg/dL)Normal diet–wild-type40.67 ± 2.1542.10 ± 3.5447.42 ± 7.20*
    ChD–wild-type82.78 ± 11.67‡88.54 ± 7.70‡98.92 ± 11.41*†‡
    ChD–apoE−/−9.44 ± 4.25‡§9.67 ± 2.15‡§11.77 ± 3.27‡§

    • ↵* P < 0.05 vs. 10-wk-old group.

    • ↵† P < 0.05 vs. 18-wk-old group.

    • ↵‡ P < 0.05 vs. normal diet–wild-type group.

    • ↵§ P < 0.05 vs. ChD–wild-type group.

  • TABLE 2

    Blood Glucose Levels in Group Given 18F-FDG

    Glucose level (mg/dL, mean ± SD) in mice that were:
    Mouse group10 wk old18 wk old25 wk old
    Normal diet–wild-type141.67 ± 36.05119.00 ± 11.47121.00 ± 11.71
    ChD–wild-type122.67 ± 20.91118.71 ± 26.23139.53 ± 16.06
    ChD–apoE−/−124.89 ± 42.59122.83 ± 17.83113.21 ± 18.0*

    • ↵* P < 0.05 vs. ChD–wild-type group.

  • TABLE 3

    Aortic Tracer Uptake Levels

    Aortic uptake level [(%ID × kg)/g, mean ± SD] in mice that were:
    TracerMouse group10 wk old18 wk old25 wk old
    18F-FDGNormal diet–wild-type0.054 ± 0.0090.062 ± 0.0080.053 ± 0.014
    ChD–wild-type0.067 ± 0.0190.099 ± 0.018*†0.164 ± 0.093*†‡
    ChD–apoE−/−0.064 ± 0.0160.145 ± 0.057*†0.249 ± 0.124*†‡
    99mTc-annexin A5Normal diet–wild-type0.021 ± 0.0030.030 ± 0.005*0.023 ± 0.003‡
    ChD–wild-type0.026 ± 0.004†0.037 ± 0.007*0.036 ± 0.005*†
    ChD–apoE−/−0.029 ± 0.007†0.042 ± 0.006*0.047 ± 0.012*†§

    • ↵* P < 0.05 vs. 10-wk-old group.

    • ↵† P < 0.05 vs. normal diet–wild-type group.

    • ↵‡ P < 0.05 vs. 18-wk-old group.

    • ↵§ P < 0.05 vs. ChD–wild-type group.

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