Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
  • Subscriptions
    • Subscribers
    • Rates
    • Journal Claims
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Permissions
    • Advertisers
    • Continuing Education
    • Corporate & Special Sales
  • About
    • About Us
    • Editorial Board
    • Editorial Contact
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • Log out
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • Log out
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
  • Subscriptions
    • Subscribers
    • Rates
    • Journal Claims
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Permissions
    • Advertisers
    • Continuing Education
    • Corporate & Special Sales
  • About
    • About Us
    • Editorial Board
    • Editorial Contact
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • Follow SNMMI on Twitter
  • Visit SNMMI on Facebook
Research ArticleCardiovascular

Folate Receptor β–Targeted PET Imaging of Macrophages in Autoimmune Myocarditis

Arghavan Jahandideh, Sauli Uotila, Mia Ståhle, Jenni Virta, Xiang-Guo Li, Ville Kytö, Päivi Marjamäki, Heidi Liljenbäck, Pekka Taimen, Vesa Oikonen, Jukka Lehtonen, Mikko I. Mäyränpää, Qingshou Chen, Philip S. Low, Juhani Knuuti, Anne Roivainen and Antti Saraste
Journal of Nuclear Medicine November 2020, 61 (11) 1643-1649; DOI: https://doi.org/10.2967/jnumed.119.241356
Arghavan Jahandideh
1Turku PET Centre, University of Turku, Turku, Finland
2Turku PET Centre, Turku University Hospital, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sauli Uotila
1Turku PET Centre, University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mia Ståhle
1Turku PET Centre, University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jenni Virta
1Turku PET Centre, University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiang-Guo Li
1Turku PET Centre, University of Turku, Turku, Finland
3Turku PET Centre, Åbo Akademi University, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ville Kytö
4Heart Center, Turku University Hospital and University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Päivi Marjamäki
1Turku PET Centre, University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Heidi Liljenbäck
1Turku PET Centre, University of Turku, Turku, Finland
5Turku Center for Disease Modeling, University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pekka Taimen
6Institute of Biomedicine, University of Turku and Department of Pathology, Turku University Hospital, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vesa Oikonen
1Turku PET Centre, University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jukka Lehtonen
7Heart and Lung Center, Helsinki University and Helsinki University Hospital, Helsinki, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mikko I. Mäyränpää
8Pathology, Helsinki University and Helsinki University Hospital, Helsinki, Finland; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qingshou Chen
9Department of Chemistry, Purdue University, West Lafayette, Indiana
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Philip S. Low
9Department of Chemistry, Purdue University, West Lafayette, Indiana
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Juhani Knuuti
1Turku PET Centre, University of Turku, Turku, Finland
2Turku PET Centre, Turku University Hospital, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne Roivainen
1Turku PET Centre, University of Turku, Turku, Finland
2Turku PET Centre, Turku University Hospital, Turku, Finland
5Turku Center for Disease Modeling, University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antti Saraste
1Turku PET Centre, University of Turku, Turku, Finland
2Turku PET Centre, Turku University Hospital, Turku, Finland
4Heart Center, Turku University Hospital and University of Turku, Turku, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF
Loading

Visual Abstract

Figure1
  • Download figure
  • Open in new tab
  • Download powerpoint

Abstract

Currently available imaging techniques have limited specificity for the detection of active myocardial inflammation. Aluminum 18F-labeled 1,4,7-triazacyclononane-N,N′,N″-triacetic acid conjugated folate (18F-FOL) is a PET tracer targeting folate receptor β (FR-β), which is expressed on activated macrophages at sites of inflammation. We evaluated 18F-FOL PET for the detection of myocardial inflammation in rats with autoimmune myocarditis and studied the expression of FR-β in human cardiac sarcoidosis specimens. Methods: Myocarditis was induced by immunizing rats (n = 18) with porcine cardiac myosin in complete Freund adjuvant. Control rats (n = 6) were injected with Freund adjuvant alone. 18F-FOL was intravenously injected, followed by imaging with a small-animal PET/CT scanner and autoradiography. Contrast-enhanced high-resolution CT or 18F-FDG PET images were used for coregistration. Rat tissue sections and myocardial autopsy samples from 6 patients with cardiac sarcoidosis were studied for macrophages and FR-β. Results: The myocardium of 10 of 18 immunized rats showed focal macrophage-rich inflammatory lesions, with FR-β expression occurring mainly in M1-polarized macrophages. PET images showed focal myocardial 18F-FOL uptake colocalizing with inflammatory lesions (SUVmean, 2.1 ± 1.1), whereas uptake in the remote myocardium of immunized rats and controls was low (SUVmean, 0.4 ± 0.2 and 0.4 ± 0.1, respectively; P < 0.01). Ex vivo autoradiography of tissue sections confirmed uptake of 18F-FOL in myocardial inflammatory lesions. Uptake of 18F-FOL in inflamed myocardium was efficiently blocked by a nonlabeled FR-β ligand folate glucosamine in vivo. The myocardium of patients with cardiac sarcoidosis showed many FR-β–positive macrophages in inflammatory lesions. Conclusion: In a rat model of autoimmune myocarditis, 18F-FOL shows specific uptake in inflamed myocardium containing macrophages expressing FR-β, which were also present in human cardiac sarcoid lesions. Imaging of FR-β expression is a potential approach for the detection of active myocardial inflammation.

  • folate receptor
  • PET
  • myocarditis
  • experimental autoimmune myocarditis

Footnotes

  • Published online Apr. 13, 2020.

  • © 2020 by the Society of Nuclear Medicine and Molecular Imaging.
View Full Text

This article requires a subscription to view the full text. If you have a subscription you may use the login form below to view the article. Access to this article can also be purchased.

SNMMI members

SNMMI Member Login

Login to the site using your SNMMI member credentials

Individuals

Non-Member Login

Login as an individual user

PreviousNext
Back to top

In this issue

Journal of Nuclear Medicine: 61 (11)
Journal of Nuclear Medicine
Vol. 61, Issue 11
November 1, 2020
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Folate Receptor β–Targeted PET Imaging of Macrophages in Autoimmune Myocarditis
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
Folate Receptor β–Targeted PET Imaging of Macrophages in Autoimmune Myocarditis
Arghavan Jahandideh, Sauli Uotila, Mia Ståhle, Jenni Virta, Xiang-Guo Li, Ville Kytö, Päivi Marjamäki, Heidi Liljenbäck, Pekka Taimen, Vesa Oikonen, Jukka Lehtonen, Mikko I. Mäyränpää, Qingshou Chen, Philip S. Low, Juhani Knuuti, Anne Roivainen, Antti Saraste
Journal of Nuclear Medicine Nov 2020, 61 (11) 1643-1649; DOI: 10.2967/jnumed.119.241356

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Folate Receptor β–Targeted PET Imaging of Macrophages in Autoimmune Myocarditis
Arghavan Jahandideh, Sauli Uotila, Mia Ståhle, Jenni Virta, Xiang-Guo Li, Ville Kytö, Päivi Marjamäki, Heidi Liljenbäck, Pekka Taimen, Vesa Oikonen, Jukka Lehtonen, Mikko I. Mäyränpää, Qingshou Chen, Philip S. Low, Juhani Knuuti, Anne Roivainen, Antti Saraste
Journal of Nuclear Medicine Nov 2020, 61 (11) 1643-1649; DOI: 10.2967/jnumed.119.241356
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Visual Abstract
    • Abstract
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • CONCLUSION
    • DISCLOSURE
    • Acknowledgments
    • Footnotes
    • REFERENCES
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF

Related Articles

  • This Month in JNM
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

Cardiovascular

  • Targeted PET Imaging of Chemokine Receptor 2–Positive Monocytes and Macrophages in the Injured Heart
  • 11C-Methionine PET Identifies Astroglia Involvement in Heart–Brain Inflammation Networking After Acute Myocardial Infarction
Show more Cardiovascular

Basic

  • Preclinical Development of 18F-OF-NB1 for Imaging GluN2B-Containing N-Methyl-d-Aspartate Receptors and Its Utility as a Biomarker for Amyotrophic Lateral Sclerosis
  • 18F-SMBT-1: A Selective and Reversible PET Tracer for Monoamine Oxidase-B Imaging
  • Preclinical Characterization of the Radioimmunoconjugate 111In or 90Y-FF-21101 Against a P-Cadherin–Expressing Tumor in a Mouse Xenograft Model and a Nonhuman Primate
Show more Basic

Similar Articles

Keywords

  • folate receptor
  • PET
  • myocarditis
  • experimental autoimmune myocarditis
SNMMI

© 2021 Journal of Nuclear Medicine

Powered by HighWire