Article Figures & Data
Figures
- FIGURE 1.
In vitro incorporation of [18F]FHBG into transduced (+) and nontransduced (−) cells at 1 and 5 h. Data are average of 3 experiments ± SEM; P < 0.001 for both time points (Student t test). cpm = counts per minute.
- FIGURE 2.
HPLC radiochromatograms of [18F]FHBG (a) and its metabolites (b–d) in HT-29 tumor cells after 5-h incubation. (A) Transduced cells. (B) Nontransduced cells.
- FIGURE 3.
Blood clearance curve of 18F activity in nude mice bearing transduced tumors after intravenous injection of [18F]FHBG (semilogarithmic plot; n = 3).
- FIGURE 4.
Time–activity curve for arterial plasma from cynomolgus monkeys after intravenous injection of [18F]FHBG (semilogarithmic plot). Total plasma volume was estimated to be 2.5% of body weight in calculating %ID in plasma. Data are mean ± SEM from 2 monkeys. Error bars are smaller than symbols for most data points. Solid line is intended to guide the eye and does not represent model fit to data.
- FIGURE 5.
HPLC radiochromatogram of [18F]FHBG and its metabolites in plasma taken 60 min after injection from cynomolgus monkey. For study shown, 86% of recovered activity was on [18F]FHBG as calculated by FHBG peak area ÷ total area under radiochromatogram.
- FIGURE 6.
Distribution of 18F from [18F]FHBG in cynomolgus monkey. Images are coronal (left) and sagittal (right) 2-dimensional projections from multicouch-position scan obtained 104–140 min after intravenous injection of [18F]FHBG. Note that skeleton is not visualized, indicating that 18F uptake in bone and marrow was relatively low.
- FIGURE 7.
Biodistribution of radiolabel from [18F]FHBG and [18F]FHPG in nude mice bearing transduced tumors. (A) Uptake at 2 h after injection. (B) Uptake at 5 h after injection. (C) Tumor-to-normal ratios at 2 h after injection. (D) Tumor-to-normal ratios at 5 h after injection. Data are mean ± SEM (n = 5). *P < 0.05 for difference between FHPG and FHBG.
Tables
Tissue Nontransduced tumor model Transduced tumor model %ID/g Tumor-to-tissue ratio %ID/g Tumor-to-tissue ratio Blood 0.035 ± 0.003 3.37 ± 0.11 0.041 ± 0.002 12.12 ± 0.91 Skin 0.056 ± 0.004 2.09 ± 0.12 0.046 ± 0.004 17.12 ± 4.25 Muscle 0.034 ± 0.002 3.49 ± 0.22 0.039 ± 0.005 21.93 ± 4.09 Bone 0.243 ± 0.013 0.53 ± 0.06 0.258 ± 0.024 2.46 ± 0.33 Heart 0.037 ± 0.003 3.24 ± 0.18 0.033 ± 0.005 24.93 ± 3.75 Lung 0.055 ± 0.005 2.14 ± 0.08 0.052 ± 0.004 12.59 ± 2.29 Liver 0.007 ± 0.014 1.65 ± 0.32 0.119 ± 0.016 6.29 ± 0.89 Spleen 0.074 ± 0.006 1.62 ± 0.11 0.068 ± 0.006 10.35 ± 2.08 Pancreas 0.073 ± 0.006 1.79 ± 0.18 0.074 ± 0.006 7.96 ± 1.14 Stomach 0.062 ± 0.009 2.45 ± 0.27 0.075 ± 0.030 8.27 ± 1.23 Intestine 0.099 ± 0.008 1.47 ± 0.23 0.110 ± 0.005 4.74 ± 0.43 Kidney 0.129 ± 0.007 0.98 ± 0.12 0.116 ± 0.012 5.48 ± 0.67 Tumor 0.113 ± 0.008 0.508 ± 0.049* ↵* P < 0.05 compared with nontransduced tumor (Student t test on paired samples).
Data are mean ± SEM of 5 animals.
Tissue Nontransduced tumor model Transduced tumor model %ID/g Tumor-to-tissue ratio %ID/g Tumor-to-tissue ratio Blood 0.017 ± 0.001 1.58 ± 0.14 0.017 ± 0.001 20.71 ± 1.06 Skin 0.026 ± 0.001 1.05 ± 0.07 0.030 ± 0.001 11.52 ± 0.62 Muscle 0.017 ± 0.001 1.64 ± 0.12 0.022 ± 0.003 22.23 ± 2.41 Bone 0.225 ± 0.015 0.13 ± 0.01 0.220 ± 0.015 1.71 ± 0.15 Heart 0.020 ± 0.001 1.48 ± 0.15 0.023 ± 0.001 15.74 ± 0.99 Lung 0.031 ± 0.002 0.89 ± 0.06 0.022 ± 0.001 15.73 ± 0.93 Liver 0.047 ± 0.002 0.54 ± 0.02 0.034 ± 0.002 11.23 ± 1.01 Spleen 0.050 ± 0.003 0.55 ± 0.04 0.043 ± 0.003 9.08 ± 0.99 Pancreas 0.028 ± 0.003 1.46 ± 0.28 0.029 ± 0.001 11.49 ± 0.51 Stomach 0.050 ± 0.003 0.84 ± 0.08 0.030 ± 0.001 11.51 ± 0.68 Intestine 0.064 ± 0.005 0.47 ± 0.05 0.058 ± 0.002 5.87 ± 0.27 Kidney 0.054 ± 0.002 0.49 ± 0.03 0.048 ± 0.005 8.86 ± 1.11 Tumor 0.025 ± 0.010 0.335 ± 0.070* ↵* P < 0.001 compared with nontransduced tumor (Student t test on paired samples).
Data are mean ± SEM of 5 animals.
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