Submillimeter resolution positron emission tomography for high-sensitivity mouse brain imaging

Abstract

Positron emission tomography (PET) is a powerful molecular imaging technique that can provide functional information of living objects. However, the spatial resolution of PET imaging has been limited to around 1 mm which makes it difficult to visualize mouse brain functions in detail. Here we report an ultrahigh resolution small animal PET scanner we developed that can provide a resolution approaching 0.6 mm to visualize the mouse brain functions with unprecedented detail. Methods: The ultrahigh resolution small animal PET scanner had 52.5 mm inner diameter and 51.5 mm axial coverage. The PET scanner consisted of 4 rings each of which had 16 DOI detectors. Each DOI detector consisted of a 3-layer staggered LYSO crystal array with a pitch of 1 mm and 4×4 SiPM array. The physical performance was evaluated in accordance with the NEMA NU4 protocol. The spatial resolution was evaluated with various resolution phantoms. In vivo glucose metabolism imaging of mouse brain was performed. Results: The peak absolute sensitivity was 2.84% with an energy window of 400-600 keV. The 0.55 mm rod structure of a resolution phantom was resolved using the iterative algorithm. The in vivo mouse brain imaging with ¹⁸F-FDG showed clear identification of cortex, thalamus, and hypothalamus which were barely distinguishable in a commercial preclinical PET scanner that we used for imaging comparison. Conclusion: The developed ultrahigh resolution small animal PET scanner is a promising molecular imaging tool for neuroscience research using rodent models.