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Research ArticleFocus on Molecular Imaging

The Development and Validation of Radiopharmaceuticals Targeting Bacterial Infection

Signore Alberto, Alvaro A. Ordonez, Chanda Arjun, Gurpreet Kaur Aulakh, Nicolas Beziere, Ekaterina Dadachova, Thomas Ebenhan, Ulises Granados, Aruna Korde, Amirreza Jalilian, Wening Lestari, Archana Mukherjee, Milos Petrik, Tamer Sakr, Clara L. Santos Cuevas, Mick M. Welling, Jan Rijn Zeevaart, Sanjay K. Jain and David M. Wilson
Journal of Nuclear Medicine November 2023, 64 (11) 1676-1682; DOI: https://doi.org/10.2967/jnumed.123.265906
Signore Alberto
1Nuclear Medicine Unit, Department of Medical-Surgical Sciences and Translational Medicine, Faculty of Medicine and Psychology, University of Rome “Sapienza,” Rome, Italy;
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Alvaro A. Ordonez
2Center for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, Maryland;
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Chanda Arjun
3Radiopharmaceutical Program, Board of Radiation and Isotope Technology, Mumbai, India;
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Gurpreet Kaur Aulakh
4Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;
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Nicolas Beziere
5Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University of Tübingen, Tübingen, Germany;
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Ekaterina Dadachova
6College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;
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Thomas Ebenhan
7Nuclear Medicine, University of Pretoria, and Radiochemistry, Applied Radiation, South African Nuclear Energy Corporation, Pelindaba, South Africa;
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Ulises Granados
8Department of Nuclear Medicine, Hospital Internacional de Colombia–Fundación Cardiovascular de Colombia, Piedecuesta, Colombia;
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Aruna Korde
9Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria;
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Amirreza Jalilian
9Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria;
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Wening Lestari
10National Nuclear Energy Agency, South Tangerang, Indonesia;
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Archana Mukherjee
11Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai, India;
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Milos Petrik
12Institute of Molecular and Translational Medicine and Czech Advanced Technology and Research Institute, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic;
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Tamer Sakr
13Radioactive Isotopes and Generator Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, Egypt;
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Clara L. Santos Cuevas
14Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Mexico;
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Mick M. Welling
15Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands; and
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Jan Rijn Zeevaart
7Nuclear Medicine, University of Pretoria, and Radiochemistry, Applied Radiation, South African Nuclear Energy Corporation, Pelindaba, South Africa;
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Sanjay K. Jain
2Center for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, Maryland;
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David M. Wilson
16Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, California
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    FIGURE 1.

    d-methyl-[11C]methionine PET/MR images of 61-y-old man with bilateral hip prostheses and confirmed C. acnes infection of left hip. Left bar represents SUV color scale, and right bar represents MRI color scale. (A, B, and C) Coronal MR, PET, and PET/MR images, respectively. Arrows indicate infected joint in A and area of radiotracer uptake surrounding joint in B and C. (D–F) Axial MR, PET, and PET/MR images. Arrows depict sinus tract communicating with skin in D and regions of radiotracer uptake in E and F. (Adapted from (35).)

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    FIGURE 2.

    [18F]FDS PET study of 67-y-old man with squamous cell carcinoma of lung and K. pneumoniae pneumonia. On left, 3-dimensional minimum-intensity projection is shown, with arrow indicating [18F]FDS signal in infected tissues. On right, transverse CT, PET, and PET/CT images (from top to bottom) indicate minimal [18F]FDS signal in right-sided cancerous lesions (arrow). (Adapted from (40).)

  • FIGURE 3.
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    FIGURE 3.

    Representative planar γ-camera images of 2 mice infected with 109 colony-forming units of P. aeruginosa (A) (green arrow and circle) and S. aureus (B) (red arrow and circle) vs. contralateral thigh, injected with only hydrogel (yellow arrows and circles) as control. Images were acquired 6 h after injection of 3.7 MBq of [99mTc]-hydrazinonicotinamide polymyxin B. Radiolabeled antibiotic binds only to gram-negative bacteria, thus highlighting presence of P. aeruginosa but not of gram-positive S. aureus. (Adapted from (54).)

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Journal of Nuclear Medicine: 64 (11)
Journal of Nuclear Medicine
Vol. 64, Issue 11
November 1, 2023
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The Development and Validation of Radiopharmaceuticals Targeting Bacterial Infection
Signore Alberto, Alvaro A. Ordonez, Chanda Arjun, Gurpreet Kaur Aulakh, Nicolas Beziere, Ekaterina Dadachova, Thomas Ebenhan, Ulises Granados, Aruna Korde, Amirreza Jalilian, Wening Lestari, Archana Mukherjee, Milos Petrik, Tamer Sakr, Clara L. Santos Cuevas, Mick M. Welling, Jan Rijn Zeevaart, Sanjay K. Jain, David M. Wilson
Journal of Nuclear Medicine Nov 2023, 64 (11) 1676-1682; DOI: 10.2967/jnumed.123.265906

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The Development and Validation of Radiopharmaceuticals Targeting Bacterial Infection
Signore Alberto, Alvaro A. Ordonez, Chanda Arjun, Gurpreet Kaur Aulakh, Nicolas Beziere, Ekaterina Dadachova, Thomas Ebenhan, Ulises Granados, Aruna Korde, Amirreza Jalilian, Wening Lestari, Archana Mukherjee, Milos Petrik, Tamer Sakr, Clara L. Santos Cuevas, Mick M. Welling, Jan Rijn Zeevaart, Sanjay K. Jain, David M. Wilson
Journal of Nuclear Medicine Nov 2023, 64 (11) 1676-1682; DOI: 10.2967/jnumed.123.265906
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Keywords

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