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Research ArticleFeatured Basic Science Article
Open Access

Single-Domain Antibody Nuclear Imaging Allows Noninvasive Quantification of LAG-3 Expression by Tumor-Infiltrating Leukocytes and Predicts Response of Immune Checkpoint Blockade

Quentin Lecocq, Robin Maximilian Awad, Yannick De Vlaeminck, Wout de Mey, Thomas Ertveldt, Cleo Goyvaerts, Geert Raes, Kris Thielemans, Marleen Keyaerts, Nick Devoogdt and Karine Breckpot
Journal of Nuclear Medicine November 2021, 62 (11) 1638-1644; DOI: https://doi.org/10.2967/jnumed.120.258871
Quentin Lecocq
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Robin Maximilian Awad
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Yannick De Vlaeminck
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Wout de Mey
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Thomas Ertveldt
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Cleo Goyvaerts
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Geert Raes
2Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, Brussels, Belgium;
3Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium;
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Kris Thielemans
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Marleen Keyaerts
4In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Brussels, Belgium; and
5Nuclear Medicine Department, UZ Brussel, Brussels, Belgium
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Nick Devoogdt
4In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Brussels, Belgium; and
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Karine Breckpot
1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
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Article Information

vol. 62 no. 11 1638-1644
DOI 
https://doi.org/10.2967/jnumed.120.258871
PubMed 
33712537

Published By 
Society of Nuclear Medicine
Print ISSN 
0161-5505
Online ISSN 
2159-662X
History 
  • Received for publication October 20, 2020
  • Accepted for publication February 3, 2021
  • Published online November 1, 2021.

Article Versions

  • previous version (March 12, 2021 - 11:03).
  • previous version (March 26, 2021 - 13:39).
  • previous version (May 14, 2021 - 18:26).
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Copyright & Usage 
© 2021 by the Society of Nuclear Medicine and Molecular Imaging. Immediate Open Access: Creative Commons Attribution 4.0 International License (CC BY) allows users to share and adapt with attribution, excluding materials credited to previous publications. License: https://creativecommons.org/licenses/by/4.0/. Details: http://jnm.snmjournals.org/site/misc/permission.xhtml.

Author Information

  1. Quentin Lecocq1,
  2. Robin Maximilian Awad1,
  3. Yannick De Vlaeminck1,
  4. Wout de Mey1,
  5. Thomas Ertveldt1,
  6. Cleo Goyvaerts1,
  7. Geert Raes2,3,
  8. Kris Thielemans1,
  9. Marleen Keyaerts4,5,
  10. Nick Devoogdt*,4 and
  11. Karine Breckpot*,1
  1. 1Laboratory for Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium;
  2. 2Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, Brussels, Belgium;
  3. 3Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium;
  4. 4In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Brussels, Belgium; and
  5. 5Nuclear Medicine Department, UZ Brussel, Brussels, Belgium
  1. For correspondence or reprints, contact Nick Devoogdt (nick.devoogdt{at}vub.be) or Karine Breckpot (karine.breckpot{at}vub.be).
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Journal of Nuclear Medicine: 62 (11)
Journal of Nuclear Medicine
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November 1, 2021
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Single-Domain Antibody Nuclear Imaging Allows Noninvasive Quantification of LAG-3 Expression by Tumor-Infiltrating Leukocytes and Predicts Response of Immune Checkpoint Blockade
Quentin Lecocq, Robin Maximilian Awad, Yannick De Vlaeminck, Wout de Mey, Thomas Ertveldt, Cleo Goyvaerts, Geert Raes, Kris Thielemans, Marleen Keyaerts, Nick Devoogdt, Karine Breckpot
Journal of Nuclear Medicine Nov 2021, 62 (11) 1638-1644; DOI: 10.2967/jnumed.120.258871

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Single-Domain Antibody Nuclear Imaging Allows Noninvasive Quantification of LAG-3 Expression by Tumor-Infiltrating Leukocytes and Predicts Response of Immune Checkpoint Blockade
Quentin Lecocq, Robin Maximilian Awad, Yannick De Vlaeminck, Wout de Mey, Thomas Ertveldt, Cleo Goyvaerts, Geert Raes, Kris Thielemans, Marleen Keyaerts, Nick Devoogdt, Karine Breckpot
Journal of Nuclear Medicine Nov 2021, 62 (11) 1638-1644; DOI: 10.2967/jnumed.120.258871
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Keywords

  • cancer
  • nuclear imaging
  • single domain antibody
  • Nanobody
  • immune checkpoint
  • LAG-3
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