Article Figures & Data
Figures
- FIGURE 1.
Synthesis and characterization of 89Zr-HDL nanoparticles. (A) Radiolabeling synthesis conditions for formation of 89Zr-HDL nanoparticles. (B) Dynamic light scattering analysis of HDL nanoparticles exposed to labeling conditions. (C) Radio–high-performance liquid chromatography analysis illustrating chemical and radiochemical purity of 89Zr-HDL nanoparticles.
- FIGURE 2.
In vivo analysis of 89Zr-HDL nanoparticles, visualized by PET/CT, showing reduced accumulation in mice treated with PLX3397. (A) PET/CT images representing 89Zr-HDL nanoparticle distribution. (B) Quantification of 89Zr-HDL nanoparticle uptake determined by drawing volume of interest (VOI) over entire tumor burden. ID = injected dose. **P < 0.01.
- FIGURE 3.
Ex vivo quantification of 89Zr-HDL nanoparticles correlates with tumor-associated macrophage density. (A) Hematoxylin and eosin (H&E), autoradiography, and immunofluorescence images of tumors excised from MMTV-PyMT mice. (B) Quantification of TAMs represented as percentage of total cells. (C) Maximum accumulation of 89Zr-HDL nanoparticles within region of interest (ROI) on autoradiography; activity was normalized to injected dose for each mouse. A.U./ID = arbitrary unit per injected dose. *P < 0.05.
Additional Files
Supplemental Data
Files in this Data Supplement:
