Research ArticleOncology

Development of a SPECT Tracer to Image c-Met Expression in a Xenograft Model of Non–Small Cell Lung Cancer

Zhaoguo Han, Yadi Xiao, Kai Wang, Ji Yan, Zunyu Xiao, Fang Fang, Zhongnan Jin, Yang Liu, Xilin Sun and Baozhong Shen
Journal of Nuclear Medicine November 2018, 59 (11) 1686-1691; DOI: https://doi.org/10.2967/jnumed.117.206730

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    (A) Saturation curve for binding of 99mTc-HYNIC-cMBP to H1993 cells with Kd of 56.30 ± 2.11. (B) Cell uptake and block-uptake of 99mTc-HYNIC-cMBP in NSCLC cells. CPM = counts/min.

  • FIGURE 2.
    FIGURE 2.

    In vivo stability of 99mTc-HYNIC-cMBP as indicated by radiochemical purity in samples of plasma (A), tumor (B), liver (C), and urine (D) at 1 h. CPM = counts/min.

  • FIGURE 3.
    FIGURE 3.

    (A) Representative coronal SPECT images of H1993 and H1299 xenografts 0–4 h after injection of 99mTc-HYNIC-cMBP. Arrows = tumor; B = bladder; K = kidney. (B) Quantitative analysis of images through counts in region of interest.*P < 0.05.**P < 0.01.

  • FIGURE 4.
    FIGURE 4.

    (A) Representative coronal SPECT images for H1993 and block-H1993 xenografts at 0.5 h. Arrows = tumor; B = bladder; K = kidney. (B) Quantitative analysis of images through counts in region of interest. **P < 0.01.

  • FIGURE 5.
    FIGURE 5.

    Biodistribution of 99mTc-HYNIC-cMBP in H1993, H1299, and block-H1993 groups at 0.5 h after injection. *P < 0.05.

  • FIGURE 6.
    FIGURE 6.

    Comparison of hematoxylin and eosin staining (top), immunofluorescence staining (middle), and autoradiography images (bottom) of H1993 and H1299 tumor sections at 0.5 h after injection of 99mTc-HYNIC-cMBP. Green fluorescence indicates high expression of c-Met.

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