Article Figures & Data
Figures
- FIGURE 1.
(A) Diminished oxygen consumption (VO2) and (B) core body temperature (Tb) changes in response to adrenergic stimulation in UCP1 KO mice. Data points represent mean ± SEM of 3–5 animals per group. *P < 0.05. **P < 0.01. ****P < 0.0001.
- FIGURE 2.
(A) Representative thermal images of WT (top) and UCP1 KO (bottom) mice taken 15 min after CL 316, 243 injections. (B) Defective BAT thermogenesis in response to adrenergic stimulation in UCP1 KO mice. Data points represent mean ± SEM of 4 animals per group. *P < 0.05. ***P < 0.001.
- FIGURE 3.
(A) Representative PET/MRI data of WT and UCP1 KO mice taken 45–60 min after CL 316, 243/saline and 18F-FDG injections. White arrows in each image point to interscapular BAT. (B) Retained BAT 18F-FDG uptake in response to adrenergic stimulation in UCP1 KO mice. Bars represent mean ± SEM of 4–5 animals per group. ****P < 0.0001.
- FIGURE 4.
(A) Impaired induction of uncoupled respiration but normal (B) glycolysis and (C) 2-deoxy-3H-glucose uptake in UCP1 KO mouse primary brown adipocytes. Data points represent mean ± SEM of 4–6 cultures per group. 2-DG = 2-deoxyglucose; AA = antimycin A; DNP = dinitrophenol; dpm = dose per minute; ECAR = extracellular acidification rate; Rot = rotenone. *P < 0.05. **P < 0.01. ****P < 0.0001.
Tables
Measurement WT body weight (g) UCP1 KO body weight (g) P Indirect calorimetry 25.3 ± 0.5 (n = 3) 29.3 ± 0.3 (n = 4) 0.0006 Rectal measurement 23.8 ± 0.3 (n = 4) 27.3 ± 0.6 (n = 5) 0.0023 Thermal imaging 25.3 ± 0.5 (n = 4) 28.3 ± 0.3 (n = 4) 0.0014 PET/MRI (saline) 20.6 ± 0.2 (n = 4) 24.3 ± 0.8 (n = 4) 0.0014 PET/MRI (CL 316, 243) 20.3 ± 0.8 (n = 5) 24.5 ± 0.4 (n = 5) 0.0015 Values are mean ± SEM. P values were obtained from unpaired 2-tailed t tests.
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