TY - JOUR T1 - Dissociation Between Brown Adipose Tissue <sup>18</sup>F-FDG Uptake and Thermogenesis in Uncoupling Protein 1–Deficient Mice JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1100 LP - 1103 DO - 10.2967/jnumed.116.186460 VL - 58 IS - 7 AU - Mohammed K. Hankir AU - Mathias Kranz AU - Susanne Keipert AU - Juliane Weiner AU - Sille G. Andreasen AU - Matthias Kern AU - Marianne Patt AU - Nora Klöting AU - John T. Heiker AU - Peter Brust AU - Swen Hesse AU - Martin Jastroch AU - Wiebke K. Fenske Y1 - 2017/07/01 UR - http://jnm.snmjournals.org/content/58/7/1100.abstract N2 - 18F-FDG PET imaging is routinely used to investigate brown adipose tissue (BAT) thermogenesis, which requires mitochondrial uncoupling protein 1 (UCP1). It remains uncertain, however, whether BAT 18F-FDG uptake is a reliable surrogate measure of UCP1-mediated heat production. Methods: UCP1 knockout (KO) and wild-type (WT) mice housed at thermoneutrality were treated with the selective β3 adrenergic receptor agonist CL 316, 243 and underwent metabolic cage, infrared thermal imaging and 18F-FDG PET/MRI experiments. Primary brown adipocytes were additionally examined for their bioenergetics by extracellular flux analysis as well as their uptake of 2-deoxy-3H-glucose. Results: In response to CL 316, 243 treatments, oxygen consumption, and BAT thermogenesis were diminished in UCP1 KO mice, but BAT 18F-FDG uptake was fully retained. Isolated UCP1 KO brown adipocytes exhibited defective induction of uncoupled respiration whereas their glycolytic flux and 2-deoxy-3H-glucose uptake rates were largely unaffected. Conclusion: Adrenergic stimulation can increase BAT 18F-FDG uptake independently of UCP1 thermogenic function. ER -