PT  - JOURNAL ARTICLE
AU  - Hankir, Mohammed K.
AU  - Kranz, Mathias
AU  - Keipert, Susanne
AU  - Weiner, Juliane
AU  - Andreasen, Sille G.
AU  - Kern, Matthias
AU  - Patt, Marianne
AU  - Klöting, Nora
AU  - Heiker, John T.
AU  - Brust, Peter
AU  - Hesse, Swen
AU  - Jastroch, Martin
AU  - Fenske, Wiebke K.
TI  - Dissociation Between Brown Adipose Tissue <sup>18</sup>F-FDG Uptake and Thermogenesis in Uncoupling Protein 1–Deficient Mice
AID  - 10.2967/jnumed.116.186460
DP  - 2017 Jul 01
TA  - Journal of Nuclear Medicine
PG  - 1100--1103
VI  - 58
IP  - 7
4099  - http://jnm.snmjournals.org/content/58/7/1100.short
4100  - http://jnm.snmjournals.org/content/58/7/1100.full
SO  - J Nucl Med2017 Jul 01; 58
AB  - 18F-FDG PET imaging is routinely used to investigate brown adipose tissue (BAT) thermogenesis, which requires mitochondrial uncoupling protein 1 (UCP1). It remains uncertain, however, whether BAT 18F-FDG uptake is a reliable surrogate measure of UCP1-mediated heat production. Methods: UCP1 knockout (KO) and wild-type (WT) mice housed at thermoneutrality were treated with the selective β3 adrenergic receptor agonist CL 316, 243 and underwent metabolic cage, infrared thermal imaging and 18F-FDG PET/MRI experiments. Primary brown adipocytes were additionally examined for their bioenergetics by extracellular flux analysis as well as their uptake of 2-deoxy-3H-glucose. Results: In response to CL 316, 243 treatments, oxygen consumption, and BAT thermogenesis were diminished in UCP1 KO mice, but BAT 18F-FDG uptake was fully retained. Isolated UCP1 KO brown adipocytes exhibited defective induction of uncoupled respiration whereas their glycolytic flux and 2-deoxy-3H-glucose uptake rates were largely unaffected. Conclusion: Adrenergic stimulation can increase BAT 18F-FDG uptake independently of UCP1 thermogenic function.