Research ArticleImaging/Infection

Imaging Macrophage Accumulation in a Murine Model of Chronic Pancreatitis with 125I-Iodo-DPA-713 SPECT/CT

Catherine A. Foss, Liansheng Liu, Ronnie C. Mease, Haofan Wang, Pankaj Pasricha and Martin G. Pomper
Journal of Nuclear Medicine October 2017, 58 (10) 1685-1690; DOI: https://doi.org/10.2967/jnumed.117.189571

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    125I-iodo-DPA-713 SPECT/CT of treated (Cer) and control (vehicle) mice. Coregistered images show radiotracer uptake in inflamed pancreas (arrows) and duodenum of treated mouse but not in either tissue of control mouse. Stomach is delineated by dotted circles. Gastrointestinal uptake of radiotracer is specific for peritoneal macrophages. Scale unit is %ID/g. D = duodenum; GI = gastrointestinal tract; K = kidney.

  • FIGURE 2.
    FIGURE 2.

    Ex vivo biodistribution in 4 treated (cerulein) and 4 control (vehicle) mice injected intravenously with 259 kBq (7 μCi) of 125I-iodo-DPA-713. The indicated tissues were collected and weighed, and their radioactivity was counted along with a standard dose for comparison. Data are mean ± SD. *P < 0.05.

  • FIGURE 3.
    FIGURE 3.

    18F-FDG PET/CT of treated (cerulein) and control (saline) mice. Coregistered images show radiotracer uptake in inflamed pancreas (solid arrow) of cerulein-treated mouse, with reduced background uptake (dashed arrow) seen in control mouse. Prominent uptake is also apparent in skeletal muscle, heart, and diaphragm. Scale unit is SUV (%ID/g/body weight). D = diaphragm; H = heart; S = skeletal muscle.

  • FIGURE 4.
    FIGURE 4.

    Ex vivo near-infrared fluorescence imaging of DPA-713-IRDye800CW in treated (cerulein) and control (saline) mice. Fluorescent tracer uptake is seen in pancreas, gastrointestinal tract (arrow), and urinary bladder (dotted circle) of treated mouse but in only urinary bladder of control mouse. Adjacent stomach is labeled for context. Color bar is relative fluorescence units (RFU). L = liver; P = pancreas; S = stomach.

  • FIGURE 5.
    FIGURE 5.

    Ex vivo near-infrared fluorescence microscopy of DPA-713-IRDye800CW in pancreatic sections from mice in Figure 4. Separate and merged images are shown for treated mouse, whereas only merged image is shown for control mouse (vehicle). Treated pancreas has abundant fluorescent tracer uptake (magenta) in CD68-positive phagocytic cells (yellow) along with abundant TSPO expression (white), whereas control pancreas has almost no phagocytic cells present, small amounts of TSPO expression, and no visible fluorescent probe uptake. Scale bar = 50 μm. Ab = antibody.

Additional Files

Back to top

In this issue

Print
Download PDF
Article Alerts
Email Article
Citation Tools
Bookmark this article

Jump to section

Keywords