Analysis of ¹⁷⁷Lu-DOTA-Octreotate Therapy–Induced DNA Damage in Peripheral Blood Lymphocytes of Patients with Neuroendocrine Tumors

Kinetics of γ-H2AX foci formation in PBLs. (A) Representative confocal images of γ-H2AX foci (green) and nuclei (red, DNA) of patient P10.5 after administration of 8,362 MBq of LuTate. Values correspond to average number of foci per cell ± SEM. (B) Number of foci per cell in individual patients measured at baseline (BL) before therapy and 10, 20, and 30 min and at 1, 2, 4, 24, 48, and 72 h after LuTate administration in one or more cycles of therapy.

Analysis of γ-H2AX foci formation in PBLs. (A) Number of foci per cell vs. time after PRRT for individual patient. Black horizontal bar and error bars represent mean ± SEM of 14–16 samples. (B) Fraction of cells with no foci (blue bars), ≤3 foci (red bars), and ≥4 foci (green bars) (mean ± SEM, n = 14–16). (C) Simulation of number of foci per cell as function of time after PRRT. Symbols represent average experimental values across all patients ± SD. Simulation was implemented for radiation dose of 42 mGy delivered as pulse (blue line) or accumulated (red line) within 80-h interval. Accumulated dose was calculated according to LuTate kinetics in blood (black line). *P < 0.05, Mann–Whitney test, for baseline vs. other time points. **P < 0.01, Mann–Whitney test, for baseline vs. other time points.