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Research ArticleBasic Science Investigations

A Modular Labeling Strategy for In Vivo PET and Near-Infrared Fluorescence Imaging of Nanoparticle Tumor Targeting

Carlos Pérez-Medina, Dalya Abdel-Atti, Yachao Zhang, Valerie A. Longo, Chrisopher P. Irwin, Tina Binderup, Jesús Ruiz-Cabello, Zahi A. Fayad, Jason S. Lewis, Willem J.M. Mulder and Thomas Reiner
Journal of Nuclear Medicine October 2014, 55 (10) 1706-1711; DOI: https://doi.org/10.2967/jnumed.114.141861
Carlos Pérez-Medina
1Centro de Investigación en Red de Enfermedades Respiratorias, Madrid, Spain
2Centro Nacional de Investigaciones Cardiovasculares, CNIC, Madrid, Spain
3Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, New York
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Dalya Abdel-Atti
4Radiochemistry and Imaging Sciences Service, Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York
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Yachao Zhang
4Radiochemistry and Imaging Sciences Service, Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York
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Valerie A. Longo
4Radiochemistry and Imaging Sciences Service, Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York
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Chrisopher P. Irwin
4Radiochemistry and Imaging Sciences Service, Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York
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Tina Binderup
3Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, New York
5Cluster for Molecular Imaging, Faculty of Health Sciences and Department of Clinical Physiology, Nuclear Medicine and PET, Copenhagen, Denmark
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Jesús Ruiz-Cabello
1Centro de Investigación en Red de Enfermedades Respiratorias, Madrid, Spain
2Centro Nacional de Investigaciones Cardiovasculares, CNIC, Madrid, Spain
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Zahi A. Fayad
3Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, New York
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Jason S. Lewis
4Radiochemistry and Imaging Sciences Service, Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York
6Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center, New York, New York; and
7Center for Molecular Imaging and Nanotechnology, Memorial Sloan-Kettering Cancer Center, New York, New York
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Willem J.M. Mulder
3Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, New York
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Thomas Reiner
4Radiochemistry and Imaging Sciences Service, Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York
7Center for Molecular Imaging and Nanotechnology, Memorial Sloan-Kettering Cancer Center, New York, New York
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  • FIGURE 1.
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    FIGURE 1.

    (A and B) Strategies for labeling of liposomes with 89Zr by click labeling (A) and surface chelation (B). (C) Lipid composition of liposomes used in present work. PBS = phosphate-buffered saline.

  • FIGURE 2.
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    FIGURE 2.

    (A and B) Size-exclusion chromatograms showing radioactive traces of 89Zr-CLL and 89Zr-9 (A) and 89Zr-SCL and 89Zr-oxalate (B). (C) In vitro serum stability of 89Zr-CLL and 89Zr-SCL.

  • FIGURE 3.
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    FIGURE 3.

    (A) Pharmacokinetics of 89Zr-SCL and 89Zr-CLL (n = 3). (B) Radioactivity distribution in selected tissues of 89Zr-SCL and 89Zr-CLL (n ≥ 3). (C) PET/CT imaging of 89Zr-SCL: CT only (left), PET/CT fusion (middle), and 3-dimensional rendering of PET/CT fusion (right) at 24 h after injection.

  • FIGURE 4.
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    FIGURE 4.

    (A) Schematic of dual-labeled liposome DiIC@89Zr-SCL. (B) Whole-body NIR fluorescence imaging (excitation wavelength = 650 nm/emission wavelength = 670 nm) (left) and 3-dimensional rendering of PET/CT fusion image (right) of same animal at 24 h after administration of DiIC@89Zr-SCL. (C) Tumor sections showing autoradiography (top) and confocal microscopy at 670 nm (bottom). (D) Comparison of NIR fluorescence (NIRF) and PET quantification measurements in tumor and skin areas (skin-to-muscle ratio for PET; n = 3).

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Journal of Nuclear Medicine: 55 (10)
Journal of Nuclear Medicine
Vol. 55, Issue 10
October 1, 2014
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A Modular Labeling Strategy for In Vivo PET and Near-Infrared Fluorescence Imaging of Nanoparticle Tumor Targeting
Carlos Pérez-Medina, Dalya Abdel-Atti, Yachao Zhang, Valerie A. Longo, Chrisopher P. Irwin, Tina Binderup, Jesús Ruiz-Cabello, Zahi A. Fayad, Jason S. Lewis, Willem J.M. Mulder, Thomas Reiner
Journal of Nuclear Medicine Oct 2014, 55 (10) 1706-1711; DOI: 10.2967/jnumed.114.141861

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A Modular Labeling Strategy for In Vivo PET and Near-Infrared Fluorescence Imaging of Nanoparticle Tumor Targeting
Carlos Pérez-Medina, Dalya Abdel-Atti, Yachao Zhang, Valerie A. Longo, Chrisopher P. Irwin, Tina Binderup, Jesús Ruiz-Cabello, Zahi A. Fayad, Jason S. Lewis, Willem J.M. Mulder, Thomas Reiner
Journal of Nuclear Medicine Oct 2014, 55 (10) 1706-1711; DOI: 10.2967/jnumed.114.141861
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Keywords

  • liposomes
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