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Figures
- FIGURE 1.
Results of oil red O staining. Cells were treated with acetylated LDL for 0 h (A), 12 h (B), 24 h (C), or 48 h (D). Moderate staining of macrophages at 24 h (pale red) and strong staining at 48 h (deep red) indicates foam cell formation.
- FIGURE 2.
18F-FDG uptake by macrophages and SMCs. Macrophages were treated with acetylated LDL for 12, 24, or 48 h. As control, PBS was added instead of acetylated LDL. Significantly higher uptake was observed under 24-h acetylated LDL–treated condition than under 24-h control condition (*P < 0.05). Uptake was significantly lower in 48-h culture than in 12- and 24-h culture under both acetylated LDL–treated and control conditions (†P < 0.05). Uptake by SMCs was significantly lower than uptake by 12- or 24-h PBS- or acetylated LDL–treated macrophages (‡P < 0.05) but was almost at same level as that of 48-h treated groups. acLDL = acetylated low-density lipoprotein; %ID = percentage injected dose.
- FIGURE 3.
Changes in hexokinase activity (A), G6Pase activity (B), and GLUT-1 expression (C and D) by acetylated LDL treatments. For western blot analysis, MCF7 cell lysate was used as molecular-weight marker for GLUT-1 and actin was used as loading control (D). Elevated hexokinase activity was observed after 24 h of acetylated LDL treatment, compared with control condition, and changes in hexokinase activity tended to follow changes in 18F-FDG uptake. G6Pase activity and GLUT-1 expression were independent of 18F-FDG uptake. acLDL = acetylated low-density lipoprotein; Ctr = control.
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