Research ArticleBasic Science Investigation

Putative Transport Mechanism and Intracellular Fate of Trans-1-Amino-3-18F-Fluorocyclobutanecarboxylic Acid in Human Prostate Cancer

Hiroyuki Okudaira, Naoto Shikano, Ryuichi Nishii, Tohru Miyagi, Mitsuyoshi Yoshimoto, Masato Kobayashi, Kazuyo Ohe, Takeo Nakanishi, Ikumi Tamai, Mikio Namiki and Keiichi Kawai
Journal of Nuclear Medicine May 2011, 52 (5) 822-829; DOI: https://doi.org/10.2967/jnumed.110.086074

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    Comparison of 14C-FACBC, 14C-Ala, 14C-Leu, and 14C-Met uptake by DU145 cells. Data are expressed as percentage of uptake in cells that were incubated without inhibitors. Uninhibited uptake of 14C-FACBC, 14C-Ala, 14C-Leu, and 14C-Met was 6,510.71, 3,086.29, 3,193.04, and 4,036.06 pmol/mg of protein, respectively. Each bar represents mean and SD (n = 3–6). *P < 0.05. **P < 0.01.

  • FIGURE 2.
    FIGURE 2.

    mRNA expression levels of amino acid transporters in human prostate biopsy specimens (A) and DU145 cells (B) determined by qRT-PCR. Each bar represents mean and SD (n = 3). rRNA = ribosomal RNA.

  • FIGURE 3.
    FIGURE 3.

    Correlation between log ratio of amino acid transporters in human prostate biopsy specimens determined by cDNA microarray and qRT-PCR. **P < 0.01.

  • FIGURE 4.
    FIGURE 4.

    Effects of ASCT2 or SNAT2 knockdown, compared with control siRNA-transfected cells. (A) mRNA expression levels of amino acid transporters in siRNA-treated DU145 cells. (B) Uptake of 14C-FACBC, 14C-Ala, 14C-Leu, and 14C-Met by each siRNA-transfected DU145 cell. Each bar represents mean and SD (n = 6). **P < 0.01.

  • FIGURE 5.
    FIGURE 5.

    Intracellular distribution of 14C-FACBC (A) and 14C-Met (B) when DU145 cells were incubated with each tracer for 3 or 24 h. Each bar represents mean and SD (n = 3), except 14C-FACBC for 3 h, which represents mean (n = 2).

Tables

  • TABLE 1

    List of Human Prostate Biopsy Specimens

    Patient no.Prostate-specific antigen (ng/mL)Gleason score
    Normal prostate
     13.6—
     25.1—
     39.0—
    Adenocarcinoma
     455.04 + 4 = 8
     596.04 + 4 = 8
     6227.84 + 4 = 8

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