Article Figures & Data
Figures
- FIGURE 1.
Effects of FN on 18F-FDG uptake, GLUT1 expression, and hexokinase activity. (A) 18F-FDG uptake in HUVEC treated with fresh or reused FN relative to that in nontreated control cells. Data are mean ± SD of 3 independent experiments. (B) Representative Western blot of plasma membrane GLUT1 protein in FN-treated (FN+) and non–FN-treated (FN−) cells. (C) Total cellular hexokinase activity relative to that in control cells. Data are mean ± SD of 2 independent experiments.
- FIGURE 2.
Effect of FAK silencing by siRNA transfection on p-FAK levels (A) and 18F-FDG uptake (B). 18F-FDG uptake data are mean ± SD of 2 independent experiments.
- FIGURE 3.
Western blot and band intensity of p-PI3K (A) and p-Akt (B) in FN-treated (FN+) and non–FN-treated (FN−) cells. Data are mean ± SD of band densities relative to that in control cells in triplicate samples. *P < 0.05 compared with control cell values. †P < 0.001 compared with control cell values.
- FIGURE 4.
Effects of specific PI3K inhibitors (A) and Akt inhibitor (B) on FN-stimulated 18F-FDG uptake. Data are mean ± SD of 3 independent experiments. AKT Inh, 1 μM Akt inhibitor IV; Ly294002, 10 μM LY294002; WM, 200 nM wortmannin.
- FIGURE 5.
Role of NO in FN-stimulated 18F-FDG uptake. (A) Effect of 10 and 100 μM SNP on 18F-FDG uptake in FN-treated (FN+) and non–FN-treated (FN−) cells. (B) Concentration of released NO in culture medium with or without addition of SNP. (C) Effect of inhibition of NO synthase by L-NAME on 18F-FDG uptake. 18F-FDG uptake data are mean ± SD of 2 independent experiments. ‡P < 0.0001 compared with control cell values.
