PT  - JOURNAL ARTICLE
AU  - Jin-Young Paik
AU  - Bong-Ho Ko
AU  - Kyung-Ho Jung
AU  - Kyung-Han Lee
TI  - Fibronectin Stimulates Endothelial Cell <sup>18</sup>F-FDG Uptake Through Focal Adhesion Kinase–Mediated Phosphatidylinositol 3-Kinase/Akt Signaling
AID  - 10.2967/jnumed.108.059386
DP  - 2009 Apr 01
TA  - Journal of Nuclear Medicine
PG  - 618--624
VI  - 50
IP  - 4
4099  - http://jnm.snmjournals.org/content/50/4/618.short
4100  - http://jnm.snmjournals.org/content/50/4/618.full
SO  - J Nucl Med2009 Apr 01; 50
AB  - There has been recent interest in the relationship between 18F-FDG uptake and the angiogenic activity of endothelial cells (ECs). The angiogenic process is strongly dependent on the interaction of ECs with matrix fibronectin (FN), a key regulator of EC survival, migration, and proliferation. Therefore, we investigated how FN influences EC glucose uptake and elucidated the signaling pathways that mediate this effect. Methods: Human umbilical vein ECs were allowed to adhere to FN-coated plates and were compared with control cells for 18F-FDG uptake, membrane GLUT1 levels, and hexokinase activity. The roles of focal adhesion kinase (FAK), phosphatidylinositol 3-kinase (PI3K), and Akt were evaluated with Western blotting, small interfering RNA (siRNA), and specific inhibitors. Results: FN adhesion significantly enhanced the protein-corrected 18F-FDG uptake in HUVEC, to 2.1-, 2.7-, and 4.3-fold that in control cells by 2, 3, and 5 d, respectively. This effect was mediated by the upregulation of both membrane GLUT1 expression and hexokinase activity and was accompanied by FAK activation. Silencing of FAK signaling by siRNA completely abrogated both FN-induced FAK phosphorylation and 18F-FDG uptake. FN also activated PI3K and Akt, well-known angiogenesis mediators, and the inhibition of either pathway totally abolished the effect of FN on 18F-FDG uptake. Nitric oxide, a downstream Akt effector that stimulates glucose uptake, was not involved in the metabolic effect of FN. Conclusion: The results of this study demonstrated that an EC–FN interaction induces strong enhancement of 18F-FDG uptake through the upregulation of GLUT1 expression and hexokinase activity. The findings also showed that the response occurs through FAK-mediated activation of PI3K and Akt, indicating a role for this pathway in modulating EC glucose metabolism.