OtherBASIC SCIENCE INVESTIGATIONS

Whole-Body Evaluation of MIBG Tissue Extraction in a Mouse Model of Long-Lasting Type II Diabetes and Its Relationship with Norepinephrine Transport Protein Concentration

Claudia Kusmic, Silvia Morbelli, Cecilia Marini, Marco Matteucci, Chiara Cappellini, Elena Pomposelli, Paolo Marzullo, Antonio L'Abbate and Gianmario Sambuceti
Journal of Nuclear Medicine October 2008, 49 (10) 1701-1706; DOI: https://doi.org/10.2967/jnumed.108.054361

Article Figures & Data

Figures

  • FIGURE 1. 
    FIGURE 1. 

    Myocardial time–activity curves of MIBG, depicted on linear scale (A) and on semilog scale (B). MIBG retention values, expressed as percentage of injected dose, are shown for control (left) and diabetic (right) mice. Early (30 min) uptake was similar for both groups of animals; by contrast, tracer washout was faster in diabetes.

  • FIGURE 2. 
    FIGURE 2. 

    Liver time–activity curves of MIBG, depicted on linear scale (A) and on semilog scale (B). MIBG retention values, expressed as percentage of injected dose, are shown for control (left) and diabetic (right) mice. Early (30 min) uptake was similar for both groups of animals; by contrast, tracer washout was faster in diabetes.

  • FIGURE 3. 
    FIGURE 3. 

    Bladder accumulation of MIBG, depicted on linear scale. MIBG accumulation curves, expressed as percentage of injected dose, are shown for control and diabetic mice. Data are mean ± SEM.

  • FIGURE 4. 
    FIGURE 4. 

    Relationship between heart washout and bladder accumulation. Data from control and diabetic mice are expressed as percentage of injected dose. Data are fitted by linear regression curve (black line, r = 0.73).

Tables

  • TABLE 1

    General Characteristics of Experimental Animals

    CharacteristicControl (n = 10)Diabetes (n = 9)
    Body weight (g)32 ± 1834 ± 12
    Plasma glucose (mg/dL)137 ± 6.7232 ± 21
    Plasma glucose at 2 h after glucose loading test (mg/dL)161 ± 5368 ± 27*
    Plasma insulin (μg/L)0.9 ± 0.31.2 ± 0.30

    • ↵* P < 0.005.

    • Values are mean ± SEM; n = number of tested animals.

  • TABLE 2

    Injected Dose and Myocardial Kinetics

    ParameterControl (n = 7)Diabetes (n = 7)
    Whole-body counts at 30 min (counts/s)816 ± 92850 ± 137
    % dose in myocardium at 30 min3.09 ± 0.483.54 ± 0.78
    % myocardial washout from minute 30 to minute 12021 ± 441 ± 7*

    • ↵* P < 0.001.

    • Values are mean ± SEM; n = number of tested animals.

  • TABLE 3

    Liver Kinetics and Urinary Excretion

    ParameterControl (n = 7)Diabetes (n = 7)
    Two-hour urinary excretion (% whole-body dose)33 ± 344 ± 2*
    % dose in liver at 30 min9.02 ± 1.438.3 ± 1.06
    % liver washout from minute 30 to minute 12020 ± 441 ± 8*

    • ↵* P < 0.01.

    • Values are mean ± SEM; n = number of tested animals.

  • TABLE 4

    Analysis of NET Density

    Control (n = 7)Diabetes (n = 7)
    ParameterHeartLiverHeartLiver
    Bmax (fmol/mg of protein)244 ± 102,640 ± 76136 ± 18*2,164 ± 93*
    KD (nmol/L)9.3 ± 0.79.7 ± 1.19.4 ± 2.59.9 ± 2.1

    • ↵* P < 0.05 vs. control values.

    • Values are mean ± SEM; n = number of tested animals.

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