Article Figures & Data
Figures
- FIGURE 1.
Size-exclusion HPLC radiochromatograms of free 99mTc-labeled PO-cDNA before (trace A) and after (trace B) addition to naked PS DNA (trace B) and after addition to PS DNA as the DNA–streptavidin–Tat nanoparticle (trace C). The retention time of the nanoparticle is confirmed by UV absorbance at 280 nm (trace D). The slight shoulder in the radioactivity profile of the DNA–streptavidin–Tat nanoparticle at higher MW is evidence that the contribution of higher-order products was no more than about 10%.
- FIGURE 2.
Histogram showing average percent accumulation at 3 h (□), 7 h (▪), and 23 h (
) of 99mTc-labeled PO DNA incubated as Tat, polyarginine (PolyA), and cholesterol (CHO) nanoparticles or carrier-free nanoparticle in KB-31, KB-G2, and TCO-1 cells (n = 3; error bars indicate 1 SD). - FIGURE 3.
Histogram showing average percent accumulation at 3 h (□), 7 h (▪), and 23 h (
) of 99mTc-labeled PS DNA incubated as Tat, polyarginine (PolyA), and cholesterol (CHO) nanoparticles or carrier-free nanoparticle in KB-31, KB-G2, and TCO-1 cells (n = 3; error bars indicate 1 SD). Note that scale differs from that in Figure 2.
Tables
- TABLE 1
Biodistribution in KB-G2 Xenograft–Bearing Mice of 99mTc-Labeled PS DNA–Streptavidin–Cholesterol, PS DNA–Streptavidin–Tat, and PO DNA–Streptavidin–Tat Nanoparticles with Sacrifice at 6 Hours and 18 Hours (n = 3)
PS DNA–streptavidin– cholesterol nanoparticle PS DNA–streptavidin– Tat nanoparticle PO DNA–streptavidin– Tat nanoparticle Time (h) Tissue %ID/g SD T/B SD %ID/g SD T/B SD %ID/g SD T/B SD 6 Blood 0.099 0.013 0.147 0.015 0.046 0.004 Heart 0.137 0.023 1.38 0.180 0.206 0.010 1.42 0.156 0.053 0.006 1.14 0.032 Lung 0.300 0.052 3.12 0.022 0.532 0.107 3.64 0.697 0.124 0.033 2.72 0.967 Liver 9.13 1.50 94.2 26.6 14.7 1.44 101 16.7 2.79 0.448 59.9 4.21 Spleen 3.39 0.436 35.0 9.81 5.35 0.351 36.8 4.77 1.19 0.140 25.5 0.686 Stomach 1.01 1.23 9.68 11.7 6.27 7.50 40.0 45.7 0.122 0.024 2.62 0.288 Large intestine 0.868 0.486 8.43 3.97 0.773 0.365 5.15 2.06 0.169 0.024 3.69 0.844 Kidney 0.866 0.126 8.85 1.77 1.32 0.058 9.08 1.00 0.448 0.064 9.63 0.503 Muscle 0.026 0.003 0.264 0.068 0.048 0.003 0.332 0.044 0.013 0.001 0.273 0.036 Bone 0.399 0.016 4.09 0.761 0.662 0.069 4.51 0.214 0.101 0.016 2.20 0.542 Tumor 0.179 0.037 1.81 0.292 0.232 0.026 1.60 0.261 0.068 0.013 1.46 0.225 18 Blood 0.050 0.011 0.044 0.010 0.014 0.001 Heart 0.092 0.007 1.91 0.299 0.104 0.025 2.34 0.309 0.026 0.002 1.90 0.072 Lung 0.245 0.060 4.98 0.892 0.227 0.023 5.31 1.41 0.111 0.009 7.98 1.09 Liver 8.04 1.75 165 28.6 9.93 3.36 223 50.6 2.60 0.451 185 15.4 Spleen 2.73 0.441 47.5 16.5 2.64 0.377 48.9 7.72 1.06 0.015 88.7 14.6 Stomach 0.192 0.078 3.95 0.711 0.165 0.130 10.0 10.9 0.063 0.010 5.77 1.83 Large intestine 0.383 0.058 13.0 11.1 0.550 0.236 27.4 24.6 0.074 0.020 14.1 14.6 Kidney 0.679 0.136 13.8 0.675 0.694 0.113 15.9 2.28 0.226 0.030 16.1 0.731 Muscle 0.022 0.008 0.422 0.079 0.021 0.006 0.464 0.064 0.008 0.001 0.550 0.066 Bone 0.405 0.123 8.13 1.48 0.389 0.065 8.87 0.669 0.113 0.019 8.10 0.620 Tumor 0.123 0.030 2.48 0.290 0.127 0.018 2.94 0.509 0.039 0.006 2.75 0.315 T/B = tissue-to-blood ratio.
