Article Figures & Data
Figures
- FIGURE 1.
Labeling with 18F-FDG and in vivo detection of human and porcine progenitor cells. All P values are nonsignificant except *P < 0.05. (A) Comparison of labeling efficiency at incubation temperatures of 20°C, 28°C, and 37°C. (B) Comparison of labeling efficiency after incubation for periods of 10, 20, 30, 45, and 60 min at 37°C. (C) Elution of radiolabel by CPC at 1 and 2 h. (D) Detection of intracoronary injected 18F-FDG progenitor cells in inferolateral territory of normal heart in vivo.
- FIGURE 2.
Dynamic tracking of 18F-FDG-labeled CPC during intracoronary injection. (A) From left to right: gross, unstained specimen shows extensive inferolateral MI (image is oriented to match subsequent PET/CT images); single frame of dynamic 3D PET/CT from same animal taken immediately after intracoronary injection shows myocardial activity localized to infarct territory and border zone; single frame from same animal taken 1 h after injection shows significant decrease in regional activity when compared with postinjection image. (B) Time–activity curves for myocardium (black line) and peripheral blood (gray line) during intracoronary injection using 3-cycle balloon-occlusion technique. Balloon was inflated for 4 min during coronary injection and subsequently deflated for 4 min during each cycle. Blood samples were drawn at 1-min intervals. (C) Time–activity curves for myocardium (black line) and peripheral blood (gray line) during high-concentration, single-bolus intracoronary injection.
- FIGURE 3.
Comparison of myocardial activity (measured as percentage of total injected activity) after balloon-occlusion delivery or high-concentration, single-bolus therapy at various time points. *P < 0.05 (ANOVA) for comparison of injection techniques at any given time point.
Tables
Characteristic Group 1: balloon occlusion Group 2: single bolus P Pig body mass (kg) 42.1 ± 0.2 41.7 ± 0.5 NS Ejection fraction (%) 46.6 ± 4.2 44.9 ± 5.1 NS Left ventricular mass (g) 89.1 ± 6.1 86.5 ± 5.3 NS Infarct size (g) 14.5 ± 2.9 17.8 ± 1.9 NS Infarct size (% of LV mass) 16.3 ± 3.4 20.6 ± 2.7 NS End-diastolic volume (mL) 64.2 ± 2.9 67.3 ± 5.2 NS End-systolic volume (mL) 35.2 ± 5.8 33.8 ± 4.9 NS Cardiac output (L/min) 2.9 ± 0.1 2.7 ± 0.5 NS Stroke volume (mL) 28.7 ± 2.3 25.5 ± 2.2 NS P value denotes comparison of mean baseline values in balloon-occlusion delivery group with mean baseline values in single-bolus delivery group using ANOVA.
NS = not significant.
Animal no. Delivery strategy Baseline labeling (%) Cell viability (%) Specific activity (kBq/107 NCs) Cell bound: 1 h (%) Cell bound: 2 h (%) 1 Balloon occlusion 92 98.6 336.7 81 71 2 Balloon occlusion 91 99.1 85.1 85 80 3 Balloon occlusion 96 99.3 29.6 81 74 4 Single bolus 93 98.9 33.3 72 68 5 Single bolus 94 99.6 77.7 85 71 6 Single bolus 92 99.1 270.1 83 74 NCs = nucleated cells.
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