Article Figures & Data
Figures
- FIGURE 1.
Expression of TnI in clones of MH3924A. (A) Northern blot. (B) Western blot. Representative blots are shown. α-Tubulin or β-actin was used as internal control. bp = base pairs.
- FIGURE 2.
Proliferation and apoptosis of HUVECs cocultured with WT-MH3924A or TnI-MH3924A cells. (A) Proliferation. (B) Apoptosis. Values are mean ± SEM. *P < 0.05 vs. WT-MH3924A.
- FIGURE 3.
Tumor growth of WT-MH3924A and TnI-MH3924 tumors until 40 d after inoculation. Values are mean ± SEM. ***P < 0.001; **P < 0.01; *P < 0.05 vs. WT-MH3924A.
- FIGURE 4.
H215O PET. Representative images and time–activity curves measured in WT-MH3924A (A and C) and TnI-MH3924A (B and D) tumor; 2-dimensional acquisition. Results of modeling analysis are shown in Table 2. T = tumor.
- FIGURE 5.
Functional MRI. Representative T2-weighted images of WT-MH3924A (A) and TnI-MH3924A (B) tumor. Corresponding parametric images for A and kep are shown in C and D. Range of signal intensity for kep and A is indicated at bottom.
Tables
- TABLE 1
Histochemical Analyses of Morris Hepatomas Transplanted into ACI Rats: Apoptosis, Proliferation, Oxidative Stress, and Inflammatory Cells
Parameter WT-MH3924A TnI-MH3924A P H215O PET K1 (mL·mL tissue−1·min−1) 0.43 ± 0.04 0.32 ± 0.05 0.14 k2 (1/min) 0.73 ± 0.09 0.25 ± 0.07 0.002 DV (mL/mL tissue) 0.65 ± 0.11 1.69 ± 0.40 0.022 VB (mL·mL−1) 0.02 ± 0.01 0.016 ± 0.007 0.94 68Ga-Albumin uptake (SUV) 0.48 ± 0.14 0.47 ± 0.09 0.91 Functional MR amplitude Total 0.84 ± 0.11 0.78 ± 0.12 0.75 Center 0.42 ± 0.09 0.49 ± 0.10 0.63 Periphery 1.12 ± 0.09 1.01 ± 0.17 0.58 Muscle 0.30 ± 0.07 0.37 ± 0.07 0.41 Functional MR kep Total 9.19 ± 0.71 4.82 ± 0.79 0.003 Center 3.42 ± 1.89 3.51 ± 1.22 0.95 Periphery 12.29 ± 1.07 5.89 ± 1.34 0.006 Muscle 2.65 ± 0.22 3.35 ± 1.04 0.56 Values are expressed as mean ± SD (n = 6).
Parameter WT-MH3924A TnI-MH3924A P H215O PET K1 (mL·mL tissue−1·min−1) 0.43 ± 0.04 0.32 ± 0.05 0.14 k2 (1/min) 0.73 ± 0.09 0.25 ± 0.07 0.002 DV (mL/mL tissue) 0.65 ± 0.11 1.69 ± 0.40 0.022 VB (mL·mL−1) 0.02 ± 0.01 0.016 ± 0.007 0.94 68Ga-Albumin uptake (SUV) 0.48 ± 0.14 0.47 ± 0.09 0.91 Functional MR amplitude Total 0.84 ± 0.11 0.78 ± 0.12 0.75 Center 0.42 ± 0.09 0.49 ± 0.10 0.63 Periphery 1.12 ± 0.09 1.01 ± 0.17 0.58 Muscle 0.30 ± 0.07 0.37 ± 0.07 0.41 Functional MR kep Total 9.19 ± 0.71 4.82 ± 0.79 0.003 Center 3.42 ± 1.89 3.51 ± 1.22 0.95 Periphery 12.29 ± 1.07 5.89 ± 1.34 0.006 Muscle 2.65 ± 0.22 3.35 ± 1.04 0.56 Tumor perfusion, blood volume, amplitude, and kep were measured in WT-MH3924A (n = 5) and TnI-MH3924A (n = 6) tumors of ACI rats. Skeletal muscle tissue was used as control in MRI studies. Values are expressed as mean ± SD.
Gene Tumor ratio Cell ratio Angiogenesis associated Mast cell protease 10 59.82 0.73 Vascular chymase 11.46 0.55 Mast cell protease 8 precursor 9.68 1.27 Mast cell protease 3 5.29 0.68 VEGF-D 3.31 3.2 FGF 2.74 0.44 Apoptosis associated Leukocyte common antigen receptor (LAR) 6.87 1.22 Leukocyte common antigen-related protein 2.56 0.45 ICE-like cysteine protease (Lice) 2.55 0.6 A2b-adenosine receptor 2.49 0.81 Signal transduction and stress Glutathione S-transferase 8.47 1.81 Prolactin 6.89 1 2′,5′-Oligoadenylate synthetase 6.68 0.48 Menkes protein 6.49 0.42 C kinase substrate calmodulin-binding protein 5.7 0.99 High-affinity IgE receptor (Fc-ε-R-I) 5.66 2.78 cAMP-regulated phosphoprotein 5.03 2.63 PPAR-γ 4.24 1.15 Cholecystokinin-A receptor 3.49 1.72 Liver microsomal retinol dehydrogenase type I 3.19 0.69 Nicotinic acetylcholine receptor α-3 3.16 1.08 Extracellular calcium-sensing receptor 3.14 0.53 Megalin 3.06 0.8 γ-Crystallin D type 3.03 0.75 Glycolipid-anchored form of acetylcholinesterase 2.88 1.14 CCA3 2.85 1.22 Regeneration-associated serpin protein-1 (rasp-1) 2.76 0.9 Cytochrome P-450b 2.76 0.82 Retinol dehydrogenase type II 2.51 1.33 Prepro-complement C3 2.47 0.19 Calcium-activated potassium channel β-subunit 2.4 1.85 Guanylin 2.33 0.79 cAMP protein kinase regulatory subunit 2.33 0.45 Hsp70-3 2.3 1.08 GABA-B receptor gb2 2.29 0.83 Na+,K+-ATPase (EC 3.6.1.3) α2 subunit 2.26 0.64 Anaphylatoxin C3a receptor 2.23 2.34 Granzyme-like protein III 2.22 0.64 GABA-A receptor δ-subunit 2.21 1.5 T-cell receptor α-chain 2.17 0.65 Insulin receptor (IRS-1) 2.02 0.48 VEGF-D = vascular endothelial growth factor-D; FGF = fibroblast growth factor; ICE = interleukin 1β converting enzyme; cAMP = cyclic adenosine monophosphate; PPAR-γ = peroxisome proliferator-activated receptor γ; CCA3 = confluent 3Y1 cell-associated 3; Hsp70-3 = heat shock protein 70-3; GABA-B = γ-aminobutyric acid receptor B; GABA-A = γ-aminobutyric acid receptor A.
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