Time Course of Apoptotic Tumor Response After a Single Dose of Chemotherapy: Comparison with 99mTc-Annexin V Uptake and Histologic Findings in an Experimental Model

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FIGURE 1.
Radioactivity and rates of positively stained cells. *P < 0.05 compared with control group.

TABLE 1

Biodistribution of ^99mTc-Annexin V

Biodistribution	Time after cyclophosphamide treatment (h) (n = 5)					P (K–W)
Biodistribution		0 (control)	4	12	20	P (K–W)
Blood	0.039 ± 0.006	0.041 ± 0.005	0.041 ± 0.007	0.037 ± 0.001	NS
Tumor	0.050 ± 0.010	0.048 ± 0.008	0.052 ± 0.014	0.077 ± 0.007*	<0.001
Muscle	0.008 ± 0.001	0.011 ± 0.008	0.009 ± 0.002	0.010 ± 0.000	NS
Thymus	0.031 ± 0.004	0.041 ± 0.007	0.065 ± 0.012*	0.068 ± 0.011*	<0.001
Spleen	1.321 ± 0.183	2.111 ± 0.432*	2.410 ± 0.463*	2.355 ± 0.157*	0.001
BM	0.621 ± 0.048	1.237 ± 0.193*	1.493 ± 0.429*	2.048 ± 0.521*	<0.001
Liver	0.586 ± 0.042	0.740 ± 0.111	0.743 ± 0.204	0.669 ± 0.087	NS
Kidney	8.317 ± 0.295	6.897 ± 0.968	5.958 ± 1.452*	5.945 ± 0.583*	0.002
TBR	1.306 ± 0.217	1.197 ± 0.288	1.281 ± 0.312	2.052 ± 0.164*	<0.001
TMR	5.908 ± 0.839	5.873 ± 2.692	5.733 ± 1.690	7.528 ± 0.586*	<0.001

↵* P < 0.05 compared with control groups.
K-W = Kruskal-Wallis test; NS = not significant; BM = bone marrow; TBR = tumor-to-blood ratio; TMR = tumor-to-muscle ratio.
Data are (%ID/g) × kg (mean ± SD) except TBR and TMR.

TABLE 2

Weight of Organs

Height	Time after cyclophosphamide treatment (h) (n = 5)					P (K–W)
Height		0 (control)	4	12	20	P (K–W)
Whole body	252.4 ± 21.34	247.2 ± 17.73	251.4 ± 15.21	248.6 ± 13.52	NS
Tumor	0.964 ± 0.181	0.940 ± 0.265	1.031 ± 0.147	0.952 ± 0.130	NS
Muscle	1.041 ± 0.541	1.036 ± 0.295	1.264 ± 0.288	1.169 ± 0.102	NS
Thymus	0.522 ± 0.066	0.454 ± 0.139	0.411 ± 0.058	0.312 ± 0.066	<0.01
Spleen	0.723 ± 0.095	0.549 ± 0.094	0.433 ± 0.061	0.392 ± 0.034	<0.01
Liver	3.343 ± 0.479	3.395 ± 0.394	3.956 ± 0.828	3.446 ± 0.371	NS
Kidney	1.713 ± 0.141	1.755 ± 0.164	1.810 ± 0.138	1.838 ± 0.056	NS