OtherBasic Science Investigations

Increased MIBG Uptake After Transfer of the Human Norepinephrine Transporter Gene in Rat Hepatoma

Annette Altmann, Maria Kissel, Sabine Zitzmann, Wolfgang Kübler, Miriam Mahmut, Peter Peschke and Uwe Haberkorn
Journal of Nuclear Medicine June 2003, 44 (6) 973-980;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    Structure of recombinant retroviral vector based on MMLV. Vector is designed to transfer hNET and hygromycin resistance (hyg) activity. Coexpression is achieved by use of internal ribosomal entry site (IRES) from EMCV. Synthetic Intron (IVS) is inserted to stabilize messenger RNA. Gene expression is regulated by EF1α promoter. LTR = long terminal repeat.

  • FIGURE 2.
    FIGURE 2.

    3H-Noradrenaline uptake in SK-N-SH neuroblastoma cells, wild-type Morris hepatoma cells, and hNET-expressing Morris hepatoma cells after 10 min of incubation with 3H-noradrenaline in presence or absence of nisoxetine. Values are means ± SDs (n = 3).

  • FIGURE 3.
    FIGURE 3.

    Time dependence of 131I-MIBG uptake in wild-type (wt) Morris hepatoma cells and hNET-expressing cell line MHhNETShyg2. Values are means ± SDs (n = 3).

  • FIGURE 4.
    FIGURE 4.

    MIBG efflux from hNET-expressing cells after 1 h of incubation with 131I-MIBG. Values are means ± SDs (n = 3).

  • FIGURE 5.
    FIGURE 5.

    Scintigraphic images of tumor-bearing young male nu/nu mice subcutaneously transplanted with hNET-expressing cells (right thigh) or wild-type Morris hepatoma cells (left thigh) at 30 min (A), 1 h (B), 2 h (C), and 24 h (D) after injection of 131I-MIBG.

  • FIGURE 6.
    FIGURE 6.

    MIBG uptake in tumor tissue at different times after 131I-MIBG administration in nu/nu mice. Values are means ± SDs (n = 4).

  • FIGURE 7.
    FIGURE 7.

    131I-MIBG uptake (impulses) in hNET-expressing and wild-type Morris hepatoma cells at different times after radiotracer application in rats. Values are means ± SDs (n = 6).

Tables

  • TABLE 1

    Radioactivity in Various Tissues at 2-48 Hours After Injection of 131I-MIBG

    TissueRadioactivity (kBq/g of tissue) at the following hours after injection of 131I-MIBG:
    24162448
    Liver641.7 ± 113.1357.1 ± 73.478.9 ± 16.767.3 ± 9.924.5 ± 4.1
    Spleen291.5 ± 29.3157.8 ± 21.545.7 ± 6.846.5 ± 10.213.6 ± 2.5
    Kidneys337.4 ± 99.6298.4 ± 34.5127.6 ± 13.2117.7 ± 23.475.3 ± 1.4
    Bowels701.9 ± 263.9519.5 ± 110.3106.3 ± 25.9126.8 ± 11.926.3 ± 18.6
    Wild-type tumor41.4 ± 12.632.9 ± 10.610.8 ± 2.89.7 ± 3.82.7 ± 0.4
    hNET-expressing tumor408.2 ± 132.4366.3 ± 113.2108.2 ± 37.967.6 ± 32.525.6 ± 9.8

    • Values are means ± SDs (n = 4). Weight represents weight of tissue sample including blood.

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