OtherBasic Science Investigations

123I- or 125I-Metaiodobenzylguanidine Visualization of Brown Adipose Tissue

Chio Okuyama, Naoki Sakane, Toshihide Yoshida, Keiji Shima, Hiroyuki Kurosawa, Kenzo Kumamoto, Yo Ushijima and Tsunehiko Nishimura
Journal of Nuclear Medicine September 2002, 43 (9) 1234-1240;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    A 4-y-old boy treated for neuroblastoma. 123I-MIBG examinations were performed in winter (February). Posterior image (A) and coronal slice of SPECT image of chest (B) 6 h after injection of MIBG show hot spots on shoulder (arrow). Subsequent CT, physical examination, and tumor marker studies failed to detect any evidence of tumor at same site. (C) Next MIBG study performed in summer (August) did not show accumulation.

  • FIGURE 2.
    FIGURE 2.

    Midsagittal section of whole body (A) and 125I-MIBG autoradiogram (B). Some well-defined intense accumulations in subcutaneous soft tissue on upper back are evident (arrows).

  • FIGURE 3.
    FIGURE 3.

    Hematoxylin-eosin stain (×80) (A) and anti-UCP1 antibody stain (×80) with additional hematoxylin stain (B) of sections of tissues in which MIBG was concentrated. Tissue was composed of multilocular cells that were positive for UCP1 antibody.

  • FIGURE 4.
    FIGURE 4.

    Time course of %ID/g of 125I-MIBG in organs of control group. Measurements of accumulations are shown as mean values. %ID/g in BAT was much higher than that of liver, spleen, and WAT; at 5 and 30 min, values were same as those of heart. They showed faster washout than that of heart samples.

  • FIGURE 5.
    FIGURE 5.

    Time course of %ID/g of 125I-MIBG in organs of groups pretreated with 6-OH-DA) (A) and reserpine (B). Measurements of accumulations are shown as mean values. 125I-MIBG concentrations in BAT were reduced in both groups.

  • FIGURE 6.
    FIGURE 6.

    Effect of 6-OH-DA and reserpine on concentration of 125I-MIBG in BAT. Results for accumulation in BAT are presented as %ID/g. Mean %ID/g values + SDs are shown. *P < 0.05, **P < 0.01, ***P < 0.005 compared with controls. At 30 and 60 min after injection, %ID/g of rats treated with each drug showed significant decrease. At 5 min, reserpine-treated rats had lower accumulation (not significant).

  • FIGURE 7.
    FIGURE 7.

    Effect of CL316,243 on concentration of 123I-MIBG in BAT (A), heart (B), and WAT (C). Mean % ID/g values ± SDs are shown. *P < 0.05 compared with controls. %ID/g in BAT of CL314,243-treated group showed significant increase at 30 min, and rapid washout was seen at 60 min. In heart, no differences were found between control group and CL316,243-treated group. In WAT, concentrations in CL314,243-treated group were significantly higher than those of control group.

Tables

  • TABLE 1

    Summary of Results of Control Rats and Rats Pretreated with 6-OH-DA and Reserpine

    Tissue or organControl6-OH-DAReserpine
    5 min30 min60 min5 min30 min60 min5 min30 min60 min
    BAT6.57 ± 2.375.51 ± 0.513.09 ± 0.226.65 ± 1.443.99 ± 0.231.69 ± 0.291.80 ± 0.381.57 ± 0.651.88 ± 0.30
    WAT0.19 ± 0.050.18 ± 0.020.16 ± 0.030.19 ± 0.040.25 ± 0.070.20 ± 0.040.21 ± 0.050.22 ± 0.030.17 ± 0.02
    Heart6.41 ± 0.476.03 ± 0.695.00 ± 0.275.00 ± 0.304.18 ± 0.672.16 ± 0.255.43 ± 0.394.59 ± 0.353.9 ± 0.30
    Spleen2.45 ± 0.212.45 ± 0.212.41 ± 0.521.80 ± 0.111.16 ± 0.090.84 ± 0.031.81 ± 0.031.37 ± 0.131.19 ± 0.05
    Liver2.47 ± 0.102.31 ± 0.371.64 ± 0.062.48 ± 0.252.63 ± 0.071.70 ± 0.392.51 ± 0.052.40 ± 0.262.06 ± 0.15

    • Data are presented as %ID/g (mean ± SD).

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