Article Figures & Data
Figures
- FIGURE 1.
Typical scintigrams of 99mTc-labeled UBI 29–41 (A), hLF 1–11 (B), ciprofloxacin (C), and human polyclonal IgG (D) 1 h after injection into mice having thigh muscles infected with S. aureus.
- FIGURE 2.
Accumulation of 99mTc-labeled antimicrobial peptide UBI 29–41 in thigh muscles of mice infected with MRSA (white bars), K. pneumoniae (square-hatched bars), or fluconazole-resistant C. albicans (black bars). Furthermore, mice were injected intramuscularly with LPS (diagonally hatched bars) or heat-killed bacteria (horizontally hatched bars) 18 h before administration of tracer. Each symbol at each time point represents mean ± SEM T/NT of at least 12 animals obtained from three independent experimental settings.
- FIGURE 3.
Accumulation of 99mTc-labeled UBI 29–41 (white bars), UBI 18–35 (square-hatched bars), hLF 1–11 (horizontally hatched bars), ciprofloxacin (black bars), and IgG (checkered bars) in thigh muscles of mice infected 18 h earlier with fluconazole-resistant C. albicans. Each symbol at each time point represents mean ± SEM T/NT of at least six animals obtained from three independent experimental settings.
- FIGURE 4.
Typical scintigrams of 99mTc-labeled UBI 29–41 (A), hLF 1–11 (B), ciprofloxacin (C), and human polyclonal IgG (D) 1 h after injection into mice having thigh muscles injected with heat-killed S. aureus.
- FIGURE 5.
(A) Typical scintigram of 99mTc-labeled UBI 29–41 1 h after injection into rabbits with thigh muscles infected with S. aureus. (B) Biodistribution of 99mTc-labeled UBI 29–41 1 h after injection with lower contrast.
- FIGURE 6.
Accumulation of 99mTc-labeled UBI 29–41 in thigh muscles of rabbits injected with MRSA (white bars), K. pneumoniae (square-hatched bars), heat-killed MRSA (diagonally hatched bars), heat-killed K. pneumoniae (horizontally hatched bars), or 50 mg LPS (black bars). Each symbol at each time point represents mean ± SEM T/NT of at least three animals obtained from independent experimental settings.
Tables
- TABLE 2
Mean T/NT of Infected/Inflamed Thigh Muscles After Injection of 99mTc-Labeled Compounds
99mTc-labeled compound S. aureus and MRSA K. pneumoniae C. albicans Sterile inflammation LPS Heat-killed microorganisms UBI 29–41 3.3 ± 0.2 (21)* 2.3 ± 0.1 (21)* 2.8 ± 0.2 (14)* 1.4 ± 0.1 (12) 1.4 ± 0.1 (12) UBI 18–35 2.4 ± 0.1 (33)* 2.7 ± 0.2 (16)* 2.7 ± 0.3 (8)* 1.0 ± 0.1 (22) 1.2 ± 0.1 (18) UBI 31–38 3.7 ± 0.4 (6)* 2.0 ± 0.1 (8)* ND 1.1 ± 0.1 (6) ND hLF 1–11 2.5 ± 0.2 (12)* ND 3.4 ± 0.3 (8)* 1.1 ± 0.1 (12) 1.4 ± 0.1 (6) hLF 4–11 1.7 ± 0.2 (6) ND ND 1.6 ± 0.2 (6) ND hLF 21–31 4.1 ± 0.7 (6)* ND 2.4 ± 0.4 (6)* 1.4 ± 0.1 (6) ND HNP-1–3 2.4 ± 0.2 (32)* 1.9 ± 0.1 (22)* 3.2 ± 0.5 (6)* 1.0 ± 0.1 (6) ND Ciprofloxacin 1.8 ± 0.1 (6) 1.7 ± 0.1 (6) 2.6 ± 0.2 (15)* 2.0 ± 0.1 (8) 1.7 ± 0.2 (6) IgG 2.5 ± 0.2 (31) 2.0 ± 0.3 (22) 4.2 ± 0.3 (6)* 2.1 ± 0.2 (8) 2.0 ± 0.1 (14) ↵* T/NT is significantly higher (P < 0.05) than T/NT in mice injected with LPS or heat-killed microorganisms.
ND = not done.
Values are mean ± SEM T/NT observed at 30, 60, and 120 min after injection of tracer. The number of animals is given in parentheses.
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