Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • View or Listen to JNM Podcast
  • Visit JNM on Facebook
  • Join JNM on LinkedIn
  • Follow JNM on Twitter
  • Subscribe to our RSS feeds
Meeting ReportOral - PhysicianPharm

89Zr Labeled Anti-PD-1 Antibody PET Monitors phorbol-12-Myristate-13-Acetate (PMA) induced Modulation of Lymphoma PD-1 Expression.

Kyung-Ho Jung, Jin Hee Lee, Mina Kim, Young Seok Cho and KYUNG-HAN LEE
Journal of Nuclear Medicine May 2021, 62 (supplement 1) 64;
Kyung-Ho Jung
1Department of Nuclear Medicine, Samsung Medical Center Seoul Korea, Republic of
2Samsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University School of Medicine Seoul Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jin Hee Lee
2Samsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University School of Medicine Seoul Korea, Republic of
1Department of Nuclear Medicine, Samsung Medical Center Seoul Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mina Kim
2Samsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University School of Medicine Seoul Korea, Republic of
1Department of Nuclear Medicine, Samsung Medical Center Seoul Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Young Seok Cho
1Department of Nuclear Medicine, Samsung Medical Center Seoul Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
KYUNG-HAN LEE
2Samsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University School of Medicine Seoul Korea, Republic of
1Department of Nuclear Medicine, Samsung Medical Center Seoul Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
Loading

Abstract

64

Introduction: Blockade targeting of the programmed death 1 receptor (PD-1) immune checkpoint is demonstrating remarkable clinical efficacy in the treatment of relapsed and refractory lymphomas. However, selection of patients for this treatment requires predictive biomarkers of response that is not sufficiently provided by immunohistochemistry. We developed an 89Zr-labeled anti-PD-1 immune PET that can noninvasively monitor modulation of PD-1 expression in lymphomas.

Methods: Anti-PD-1 underwent sulfohydryl moiety-specific conjugation with maleimide-deferoxamine followed by 89Zr labeling. Murine T-cell lymphoma EL4 cells underwent binding assays and Western blotting. In vivo pharmacokinetics, biodistribution, and PET imaging were evaluated in mice.

Results: 89Zr-anti-PD-1 synthesis was efficient (radiolabel yield > 70%) and polyacrylamide gel electrophoresis displayed a clear radioactive band at 170 KDa. Instant thin-layer chromatography showed that the radiolabel was stable and > 55% intact after 6 days incubation in 50% FBS. EL4 lymphoma cells expressed PD-1 protein and avidly took up 89Zr-anti-PD-1. Treatment of the cells with 50 ng/ml PMA for 24 h augmented PD-1 expression to 5.8 ± 2.5-fold of controls and increased 89Zr-anti-PD-1 binding to 3.0 ± 0.1-fold. Excellent target specificity was confirmed by a near-complete reduction of binding by excess cold antibody. Intravenous 89Zr-anti-PD-1 followed biexponential blood clearance in mice. PET/CT demonstrated high contrast EL4 lymphoma imaging that was further enhanced following PMA treatment and suppressed by cold antibody. Biodistribution revealed that PMA treatment substantially increased EL4 tumor uptake from 6.6% ± 1.6% to 13.9% ± 3.6% injected dose per gram. Immunoblots confirmed that this correlated with significant increases of tumor PD-1 protein.

Conclusions: 89Zr-anti-PD-1 showed specific targeting with favorable imaging properties. PMA stimulation upregulated PD-1 expression and 89Zr-anti-PD-1 binding to EL4 lymphoma cells in vitro and EL4 tumors in vivo. 89Zr-anti-PD-1 PET can thus be useful for monitoring PD-1 modulation in lymphomas of living subjects.

Figure
  • Download figure
  • Open in new tab
  • Download powerpoint
Previous
Back to top

In this issue

Journal of Nuclear Medicine
Vol. 62, Issue supplement 1
May 1, 2021
  • Table of Contents
  • Index by author
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
89Zr Labeled Anti-PD-1 Antibody PET Monitors phorbol-12-Myristate-13-Acetate (PMA) induced Modulation of Lymphoma PD-1 Expression.
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
89Zr Labeled Anti-PD-1 Antibody PET Monitors phorbol-12-Myristate-13-Acetate (PMA) induced Modulation of Lymphoma PD-1 Expression.
Kyung-Ho Jung, Jin Hee Lee, Mina Kim, Young Seok Cho, KYUNG-HAN LEE
Journal of Nuclear Medicine May 2021, 62 (supplement 1) 64;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
89Zr Labeled Anti-PD-1 Antibody PET Monitors phorbol-12-Myristate-13-Acetate (PMA) induced Modulation of Lymphoma PD-1 Expression.
Kyung-Ho Jung, Jin Hee Lee, Mina Kim, Young Seok Cho, KYUNG-HAN LEE
Journal of Nuclear Medicine May 2021, 62 (supplement 1) 64;
Twitter logo Facebook logo LinkedIn logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics

Related Articles

  • No related articles found.
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

Oral - PhysicianPharm

  • Super-resolution in brain PET Using a Real Time Motion Capture System
  • Pre-selecting radiomic features based on their robustness to changes in imaging properties of multicentre data: impact on predictive modelling performance compared to ComBat harmonization of all available features
  • Spleen targeting H2S donating liposome as theranostic immune modulator in dextran sodium sulfate induced colitis model
Show more Oral - PhysicianPharm

Oncology (Science), Basic and Translation: Molecular Imaging

  • Molecular Imaging of Multiple Myeloma Targeting CD46 Using ImmunoPET
  • Robotic radioguided surgery using a DROP-IN gamma probe - technical insights for different prostate cancer indications
  • Toll-like receptor 5 in triple-negative breast cancer: a novel reporter for tumor progression
Show more Oncology (Science), Basic and Translation: Molecular Imaging

Similar Articles

SNMMI

© 2025 SNMMI

Powered by HighWire