Article Figures & Data
Figures
- FIGURE 1.
(A) In vitro binding specificity of 111In-DOTA-5D3 in PSMA(+) PC3 PIP and PSMA(−) PC3 flu cell lines at 37°C. (B) Immunoreactive fractions of 111In-DOTA-5D3 in PSMA(+) PC3 PIP cells was determined as 1/y (x = 0). (C) Cell surface PSMA expression by flow cytometry illustrated by overlapping histograms and mean fluorescence intensity (MFI) for 4 different cell lines: CHO (Chinese hampster ovary cell line with no staining with anti-PSMA mAb), PC3 flu (human PC3 with no staining with anti-PSMA mAb), PC3 PIP (human PC3 with PSMA-positive staining with anti-PSMA mAb), and LNCaP (human prostate cell line). Notice shift for no staining (CHO, gray; flu, green) and staining with PE antihuman PSMA (FOLH1) mAb (LNCaP, blue; PIP, red). PSMA PE = phycoerythrin (PE)-conjugated anti-PSMA antibody.
- FIGURE 2.
(A) Tissue biodistribution of 111In-DOTA-5D3 in male NOD-SCID mice bearing PSMA(+) PC3 PIP and PSMA(−) PC3 flu tumors on either flank (n = 4). (B) Selected PSMA(+) PC3 PIP tumor-to-normal organ ratios. (C) Binding specificity assessed by blockade with coinjection of either 10- or 50-fold excess of nonradiolabeled 5D3. (D) Immunohistochemistry of PSMA expression and negative control (no PSMA staining) of frozen tissue sections of PSMA(+) PC3 PIP tumor and PSMA(−)PC3 flu tumor at ×10 magnification obtained from biodistribution experiment at 24 h after injection of 111In-DOTA-5D3 and from blocking experiment at 24 h using 50-fold excess of 5D3.
- FIGURE 3.
Biodistribution analysis at 2 h and 24 h after injection of 111In-DOTA-5D3 in male NOD-SCID bearing PSMA(+) PC3 PIP and PSMA(−) PC3 flu tumors on either flank (n = 4) and in male CD-1 mice (n = 3).
- FIGURE 4.
Whole-body volume rendering SPECT/CT images in male NOD-SCID mice showing uptake of 11In-DOTA-5D3 in normal tissues (e.g., liver, kidneys) and PSMA(+) PC3 PIP and PSMA(−) PC3 flu tumors.
- FIGURE 5.
(A) Calculated human organ time-integrated activity coefficients. (B) Estimated human organ absorbed doses (mGy/MBq). Absorbed doses were calculated on the basis of murine biodistribution data, which were converted into adult human absorbed doses. Calculated time-integrated activities were used as input in OLINDA/EXM using adult male phantom for normal tissues.
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