Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Corporate & Special Sales
    • Journal Claims
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Permissions
    • Advertisers
    • Continuing Education
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Corporate & Special Sales
    • Journal Claims
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Permissions
    • Advertisers
    • Continuing Education
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • Follow JNM on Twitter
  • Visit JNM on Facebook
  • Join JNM on LinkedIn
  • Subscribe to our RSS feeds
Meeting ReportOncology, Basic Science Track

HER2-targeted SPECT imaging of nude mice with colon cancer xenografts using 125I-Herceptin

Tao Wang, Ye Peng, Xiao Li, Guixia Pan and Changjing Zuo
Journal of Nuclear Medicine May 2018, 59 (supplement 1) 1299;
Tao Wang
1Department of Nuclear Medicine Changhai Hospitai, Second Military Medical University Shanghai China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ye Peng
1Department of Nuclear Medicine Changhai Hospitai, Second Military Medical University Shanghai China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiao Li
1Department of Nuclear Medicine Changhai Hospitai, Second Military Medical University Shanghai China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guixia Pan
1Department of Nuclear Medicine Changhai Hospitai, Second Military Medical University Shanghai China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Changjing Zuo
1Department of Nuclear Medicine Changhai Hospitai, Second Military Medical University Shanghai China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
Loading

Abstract

1299

Aim: R2-targeted SPECT imaging of nude mice with colon cancer xenografts using 125I-Herceptin

Objectives: The overexpression of human epidermal growth factor receptor 2 (HER2) is associated with poor clinical outcomes, and has been found in many types of cancer, including breast cancer, ovarian cancer, endometrial cancer and colorectal cancer. Herceptin (Pertuzumab) was the first FDA-approved humanized monoclonal antibody for the targeted therapy of HER2+ breast cancer. Since HER2 overexpression also occurs in colorectal cancer, it is expected that 125I-labeled Herceptin targeting HER2 can provide SPECT imaging of colon cancer xenografts.

Methods: Herceptin was radiolabeled with 125I using the Iodogen

Methods: The expression level of HER2 in colon carcinoma MC38 cells was detected by Western blotting, and MC38 cells were injected subcutaneously into the nude mice for the establishment of colon cancer xenografts model. The specificity of 125I-Herceptin was assessed using the in vitro cell binding assay. The SPECT imaging studies were performed in nude mice with colon cancer xenografts at different time points post-injection. For the blocking studies, excessive Herceptin was intravenously injected into the nude mice, 24 hours prior to 125I-Herceptin administration.

Results: The labeling yield of 125I-Herceptin exceeded 98% and the radiochemical purity was greater than 95%. HER2 was expressed in MC 38 cells. 125I-Herceptin showed excellent HER2-binding specificity both in vitro and in vivo. SPECT imaging of nude mice with colon cancer xenografts using 125I-Herceptin revealed that the tumors were clearly visualized at 6 h, and the tumor uptake reached peak at 24 h after injection. In addition, the maximum uptake was reduced at 24 h time point in blocking studies suggesting specificity of 125I-Herceptin to HER2.

Conclusions: Our study suggests 125I-Herceptin can be used as an effective SPECT probe for the non-invasive detection of colon cancer expressing HER2. Research Support: This work is supported by the National Natural Science Foundation of China (Grant No. 81471714) and the Staring Foundation for Young Researcher of Changhai Hospital (Grant No. CH201714).

Figure
  • Download figure
  • Open in new tab
  • Download powerpoint
Previous
Back to top

In this issue

Journal of Nuclear Medicine
Vol. 59, Issue supplement 1
May 1, 2018
  • Table of Contents
  • Index by author
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
HER2-targeted SPECT imaging of nude mice with colon cancer xenografts using 125I-Herceptin
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
HER2-targeted SPECT imaging of nude mice with colon cancer xenografts using 125I-Herceptin
Tao Wang, Ye Peng, Xiao Li, Guixia Pan, Changjing Zuo
Journal of Nuclear Medicine May 2018, 59 (supplement 1) 1299;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
HER2-targeted SPECT imaging of nude mice with colon cancer xenografts using 125I-Herceptin
Tao Wang, Ye Peng, Xiao Li, Guixia Pan, Changjing Zuo
Journal of Nuclear Medicine May 2018, 59 (supplement 1) 1299;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics

Related Articles

  • No related articles found.
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

Oncology, Basic Science Track

  • Imaging adult glioma with 68Ga-citrate PET/MR
  • Evaluation of L-1-[18F]Fluoroethyl-Tryptophan for PET Imaging of Cancer
  • Pretargeted radioimmunotherapy with 225Ac-proteus-DOTA hapten.
Show more Oncology, Basic Science Track

Basic Science Posters (Oncology)

  • Imaging adult glioma with 68Ga-citrate PET/MR
  • Evaluation of L-1-[18F]Fluoroethyl-Tryptophan for PET Imaging of Cancer
Show more Basic Science Posters (Oncology)

Similar Articles

SNMMI

© 2022 Journal of Nuclear Medicine

Powered by HighWire