Effects of RNAi Silencing CITED1 Gene on Biological Function of Thyroid Carcinoma Cell Line K1

Purpose: Effects of RNAi Silencing CITED1 Gene on Biological Function of Thyroid Carcinoma Cell Line K1 Ying Wang¹, Hua Pang¹, Huan Huang¹, Qian Liu¹, Jia Li¹, Lei Zhang^{1 1}Department of Nuclear Medicine, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Corresponding author: Hua Pang PhD., Email: phua1973@163.com Abstract Objectives: To investigate the changes of K1 cell proliferation and apoptosis after CITED1 gene silencing.

Methods: CITED1-siRNA and negative control were successfully transfected into thyroid cancer K1 cell line. Real-time PCR and Western blot were used to detect the knock down efficiency. CCK-8 method was used to detect cell proliferation, FACS method was used to detect cell cycle, and wound healing assay was carried out, cell apoptosis was analysed by flow cytometry.

Results: Slow virus successfully infected K1 cells, mRNA and protein detection showed that the CITED1 gene was knocked down successfully. After CITED1 gene knocked down, the cell proliferation rate significantly inhibited, G_0/1 phase cells increased, G₂/M and S phase decreased, cell apoptosis rate was significantly higher, and the relative migration abilities of CITED1-siRNA group at 12, 24h after transfection were significantly lower than those in normal control group and negative control group.

Conclusions: CITED1 gene may affect the occurrence and development of thyroid papillary cancer by promoting tumor cell proliferation and migration,and inhibiting apoptosis. Key words: CITED1, K1, RNAi, apoptosis, proliferation