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Research ArticleBasic Science Investigations

Single-Cell Characterization of 18F-FLT Uptake with Radioluminescence Microscopy

Debanti Sengupta and Guillem Pratx
Journal of Nuclear Medicine July 2016, 57 (7) 1136-1140; DOI: https://doi.org/10.2967/jnumed.115.167734
Debanti Sengupta
Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, California
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Guillem Pratx
Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, California
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  • FIGURE 1.
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    FIGURE 1.

    Comparison of steps to image cells with EdU or 18F-FLT.

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    FIGURE 2.

    MDA-MB-231 cells imaged using 18F-FLT and radioluminescence microscopy or EdU and fluorescence microscopy. Red and green arrows indicate cells with negative and positive signals, respectively.

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    FIGURE 3.

    Quantification of individual cell signals from 18F-FLT (left) and EdU (right). Each dot represents an individual cell. Red and black dots represent cells below and above signal threshold, respectively. A.U. = arbitrary units.

  • FIGURE 4.
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    FIGURE 4.

    Single-cell 18F-FLT uptake, measured shortly after removal of residual 18F-FLT (x-axis) and 1 h later (y-axis). Each dot represents a single cell. Decay correction is applied. For reference, red line with slope of 1 is shown. Boxed region delineates 18F-FLT–low subpopulation.

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Journal of Nuclear Medicine: 57 (7)
Journal of Nuclear Medicine
Vol. 57, Issue 7
July 1, 2016
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Single-Cell Characterization of 18F-FLT Uptake with Radioluminescence Microscopy
Debanti Sengupta, Guillem Pratx
Journal of Nuclear Medicine Jul 2016, 57 (7) 1136-1140; DOI: 10.2967/jnumed.115.167734

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Single-Cell Characterization of 18F-FLT Uptake with Radioluminescence Microscopy
Debanti Sengupta, Guillem Pratx
Journal of Nuclear Medicine Jul 2016, 57 (7) 1136-1140; DOI: 10.2967/jnumed.115.167734
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