Research ArticleBasic Science Investigations

Immuno-PET Imaging and Radioimmunotherapy of 64Cu-/177Lu-Labeled Anti-EGFR Antibody in Esophageal Squamous Cell Carcinoma Model

In Ho Song, Tae Sup Lee, Yong Serk Park, Jin Sook Lee, Byung Chul Lee, Byung Seok Moon, Gwang Il An, Hae Won Lee, Kwang Il Kim, Yong Jin Lee, Joo Hyun Kang and Sang Moo Lim
Journal of Nuclear Medicine July 2016, 57 (7) 1105-1111; DOI: https://doi.org/10.2967/jnumed.115.167155

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Figures

  • FIGURE 1.
    FIGURE 1.

    Analysis of EGFR expression and cytotoxicity of 177Lu-labeled cetuximab on ESCC cell lines. (A) Flow cytometry using cetuximab. (B) Cell binding assay of 64Cu-/177Lu-PCTA-cetuximab. (C) Cytotoxicity of 177Lu-PCTA-cetuximab. CET = cetuximab; Con = control.

  • FIGURE 2.
    FIGURE 2.

    Immuno-PET images of 64Cu-PCTA-cetuximab in TE-4 (A) or TE-8 (B) model at each time point. White dotted circle is tumor.

  • FIGURE 3.
    FIGURE 3.

    Micro-SPECT/CT and digital whole-body autoradiography images of 177Lu-cetuximab without (A) and with (B) blocking (cetuximab 2 mg/head) in TE-8 models. SPECT/CT volume images (left first 2 panels) and coronal images (middle panels) were acquired on day 5 after injection. After micro-SPECT/CT scanning, digital whole-body autoradiography (third panels) and frozen photo (fourth panels) images were obtained.

  • FIGURE 4.
    FIGURE 4.

    Radioimmunotherapeutic efficacy of 177Lu-PCTA-cetuximab in TE-8 models. * = vs. saline- and cetuximab-treated groups, P < 0.05. a.u. = arbitrary unit.

  • FIGURE 5.
    FIGURE 5.

    Therapeutic response monitoring of 177Lu-cetuximab treatment using 18F-FDG PET in TE-8 models. (A) 18F-FDG PET images were obtained on day 7 (top) and day 14 (bottom) after treatment. (B) Quantitative 18F-FDG uptake analysis. * = vs. saline-treated group, P < 0.05. CET = cetuximab.

  • FIGURE 6.
    FIGURE 6.

    Immunohistochemial staining of TUNEL and Ki-67 in TE-8 tumors. TUNEL (A) and Ki-67 (B) staining showing positive cells were stained brown (×400 magnification). Quantitative analysis of TUNEL (C) and Ki-67 (D) staining. *P < 0.001. CET = cetuximab.

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