Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • View or Listen to JNM Podcast
  • Visit JNM on Facebook
  • Join JNM on LinkedIn
  • Follow JNM on Twitter
  • Subscribe to our RSS feeds
Research ArticleBasic Science Investigations

18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity

Esther Wolfs, Tom Struys, Tineke Notelaers, Scott J. Roberts, Abhishek Sohni, Guy Bormans, Koen Van Laere, Frank P. Luyten, Olivier Gheysens, Ivo Lambrichts, Catherine M. Verfaillie and Christophe M. Deroose
Journal of Nuclear Medicine March 2013, 54 (3) 447-454; DOI: https://doi.org/10.2967/jnumed.112.108316
Esther Wolfs
1Division of Nuclear Medicine and Molecular Imaging, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tom Struys
2Lab of Histology, Department of Functional Morphology, Biomedical Research Institute, Universiteit Hasselt, Diepenbeek, Belgium
3Biomedical NMR Unit, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tineke Notelaers
4Stem Cell Institute, Department of Development and Regeneration, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Scott J. Roberts
5Laboratory for Skeletal Development and Joint Disorders, KU Leuven, Leuven, Belgium; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Abhishek Sohni
4Stem Cell Institute, Department of Development and Regeneration, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guy Bormans
6Laboratory for Radiopharmacy, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Koen Van Laere
1Division of Nuclear Medicine and Molecular Imaging, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Frank P. Luyten
5Laboratory for Skeletal Development and Joint Disorders, KU Leuven, Leuven, Belgium; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Olivier Gheysens
1Division of Nuclear Medicine and Molecular Imaging, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ivo Lambrichts
2Lab of Histology, Department of Functional Morphology, Biomedical Research Institute, Universiteit Hasselt, Diepenbeek, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine M. Verfaillie
4Stem Cell Institute, Department of Development and Regeneration, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christophe M. Deroose
1Division of Nuclear Medicine and Molecular Imaging, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Additional Files
  • FIGURE 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 1.

    (A and B) 18F-FDG uptake kinetics in MSCs and MAPCs: data corrected for radioactive decay (A) and uncorrected data (B). (C) Cellular retention of incorporated 18F-FDG within 180 min.

  • FIGURE 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 2.

    Adipogenic, osteogenic, and chondrogenic differentiations of MSCs labeled with 18F-FDG. (A) Extraction of Oil red O dye after adipogenic differentiation measured at 490 nm. (B) Extraction of Alizarin red S (Sigma-Aldrich) dye from differentiated osteoblasts, measured at 560 nm. (C) Extraction of Alcian Blue (Sigma-Aldrich) dye from differentiated chondrocytes, measured at 595 nm. **P < 0.01.

  • FIGURE 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 3.

    Quantitative data from TEM of MSCs (A) and MAPCs (B). For both cell types, the following different parameters were assessed: number of lysosomes per cell, average lysosome size, number of vacuoles per cell, average rER width, and average mitochondrial size.

  • FIGURE 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 4.

    Small-animal PET data of injected MSCs (A–B) and MAPCs (C–D) labeled with 18F-FDG. Dynamic data from lungs and bladder suggest in vivo elution of 18F-FDG from cells for both cell types. Brain dynamic data were included as control for free 18F-FDG uptake (n = 500,000).

Additional Files

  • Figures
  • Supplemental Data

    Files in this Data Supplement:

    • Supplemental Data
PreviousNext
Back to top

In this issue

Journal of Nuclear Medicine: 54 (3)
Journal of Nuclear Medicine
Vol. 54, Issue 3
March 1, 2013
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity
Esther Wolfs, Tom Struys, Tineke Notelaers, Scott J. Roberts, Abhishek Sohni, Guy Bormans, Koen Van Laere, Frank P. Luyten, Olivier Gheysens, Ivo Lambrichts, Catherine M. Verfaillie, Christophe M. Deroose
Journal of Nuclear Medicine Mar 2013, 54 (3) 447-454; DOI: 10.2967/jnumed.112.108316

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity
Esther Wolfs, Tom Struys, Tineke Notelaers, Scott J. Roberts, Abhishek Sohni, Guy Bormans, Koen Van Laere, Frank P. Luyten, Olivier Gheysens, Ivo Lambrichts, Catherine M. Verfaillie, Christophe M. Deroose
Journal of Nuclear Medicine Mar 2013, 54 (3) 447-454; DOI: 10.2967/jnumed.112.108316
Twitter logo Facebook logo LinkedIn logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
    • Abstract
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • CONCLUSION
    • DISCLOSURE
    • Acknowledgments
    • Footnotes
    • REFERENCES
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF

Related Articles

  • This Month in JNM
  • PubMed
  • Google Scholar

Cited By...

  • Cell therapy for ARDS: efficacy of endobronchial versus intravenous administration and biodistribution of MAPCs in a large animal model
  • Google Scholar

More in this TOC Section

  • Tumor-Specific Binding of Radiolabeled PEGylated GIRLRG Peptide: A Novel Agent for Targeting Cancers
  • PET/MRI of Hypoxic Atherosclerosis Using 64Cu-ATSM in a Rabbit Model
  • Tumor Uptake of Anti-CD20 Fabs Depends on Tumor Perfusion
Show more Basic Science Investigations

Similar Articles

Keywords

  • mesenchymal stem cell
  • multipotent adult progenitor cell
  • 18F-FDG
  • radiotoxicity
  • PET
SNMMI

© 2025 SNMMI

Powered by HighWire