Article Figures & Data
Figures
- FIGURE 1.
Matched PET images from 4 representative mice used in this study. Warmer colors represent higher activity.
- FIGURE 2.
Comparison between %ID/g (A), SUV (B), and whole-brain normalized uptake (C) for 12 regions of Mirrione atlas and for each frame. Images were normalized using CT–CT registration, and results are for 6-mo group. Error bars indicate mean + SD of group.
- FIGURE 3.
SPM images (from left to right: coronal, sagittal, and horizontal axis) of reduction in uptake in frame 4, compared with frame 1 (for 6-mo CT–CT registered data). Images at top show complete spectrum of T values, images in the middle show threshold applied at level of uncorrected P < 0.001, and images at bottom show threshold applied at level of familywise error–corrected P < 0.05. SPM images are overlaid on T2 image from Mirrione atlas for anatomic reference, and same color scale (where warmest color represents T value of 20) was used for all images.
- FIGURE 4.
Surface-rendered 3-dimensional SPM images depict reduction in metabolism for 6-mo vs. 10-mo groups (left) and 6-mo vs. 14-mo groups (right). Images were registered using CT–CT method and normalized to whole-brain activity, and 1.0 mm of smoothing was applied. A surface rendering of the T2-weighted MR image is provided to aid localization.
Tables
6 mo 10 mo 14 mo Mouse no. Age (mo) Weight (g) Age (mo) Weight (g) Age (mo) Weight (g) 1 (F) 5.4 34 10.4 50 13.7 58 2 (M) 6.7 46 10.8 51 14.0 53 3 (M) 6.9 49 11.6 49 14.0 46 4 (M) 6.1 36 10.0 40 15.3 38 5 (M) 6.1 43 10.7 43 13.4 48 6 (M) 6.4 32 11.0 41 13.7 42 7 (F) 6.1 24 10.3 28 15.3 30 8 (M) 5.8 32 10.2 38 13.3 37 9 (F) 7.1 28 11.8 32 14.4 37 10 (M) 5.8 32 10.0 35 15.3 42 11 (M) 6.1 32 10.0 31 15.3 35 12 (F) 6.6 29 10.2 39 15.3 55 Average 6.3 34.8 10.6 39.8 14.4 43.4 SD 0.5 7.5 0.6 7.6 0.8 8.7 - TABLE 2
Maximum T Scores in Various Brain Regions from SPM Analysis of Reduction in Metabolism from 6 to 10 Months
CT–CT PET–PET Region SUV Norm SUV Norm Whole brain 2.94 8.40 2.70 5.56 Caudal brain stem 2.94 8.40 2.42 4.15 Cerebellum 2.90 5.69 2.60 5.10 Striatum 2.62 5.79 2.41 3.55 Midbrain 2.59 5.97 2.44 5.56 Cortex 2.58 6.00 2.70 4.84 Thalamus 2.56 6.28 2.41 4.90 Hippocampus 2.55 5.50 2.40 4.75 Amygdala 2.44 2.70 2.22 0.00 Hypothalamus 2.37 2.97 2.16 0.69 Basal forebrain and septum 2.35 2.27 2.26 0.17 Olfactory bulb 2.15 0.08 2.56 2.19 Scan length = 40 min; smoothing = 1.0 mm.
Data are for images registered using CT–CT and PET–PET and for images scaled to units of SUV and normalized to whole-brain activity.
- TABLE 3
Maximum T Scores in Caudal Brain Stem for 2 Age Comparisons with Different Scan Lengths and Smoothing Levels
Level of smoothing CT–CT PET–PET Scan length 0.0 0.5 1.0 1.5 0.0 0.5 1.0 1.5 6–10 mo 10 min* 7.85 7.69 7.07 6.11 4.94 4.80 4.42 3.66 20 min* 7.70 7.22 7.06 6.17 4.15 4.11 3.97 3.66 30 min* 7.51 7.90 8.48 6.59 4.74 4.05 3.67 3.48 40 min* 8.47 9.00 8.40 6.10 4.12 4.18 4.15 3.92 10–14 mo 10 min* 6.53 6.48 6.64 6.87 4.16 4.17 4.10 3.65 20 min* 6.27 6.57 6.34 6.40 5.78 5.43 4.51 3.90 30 min* 6.78 6.71 6.36 6.79 5.89 5.92 5.10 4.32 40 min* 6.74 6.83 6.83 7.04 5.19 5.22 4.96 4.46 ↵* Significant difference (P < 0.05) between CT–CT and PET–PET, based on paired t test over 4 levels of smoothing.
- TABLE 4
Maximum T Scores in Various Brain Regions from SPM Analysis of Reduction in Metabolism with Age for Various Scan Lengths and Smoothing Levels
Level of smoothing 6–10 mo* 10–14 mo Region 0.0 0.5 1.0 1.5 0.0 0.5 1.0 1.5 10 min Caudal brain stem 7.85 7.69 7.07 6.11 6.53 6.48 6.64 6.87 Cerebellum 7.08 6.38 7.19 6.55 6.11 5.85 7.20 8.09 Striatum 5.27 5.04 5.09 4.80 3.52 3.70 3.93 3.53 Midbrain 8.88 8.72 8.35 7.31 3.72 3.19 2.94 3.17 Cortex 7.01 6.50 5.61 4.92 4.25 3.81 4.46 5.25 20 min Caudal brain stem 7.70 7.22 7.06 6.17 6.27 6.57 6.34 6.40 Cerebellum 6.97 6.74 6.22 5.27 7.15 6.95 7.49 8.24 Striatum 5.68 5.60 4.88 4.50 4.21 4.32 4.06 3.30 Midbrain 8.47 8.32 7.39 6.08 2.58 2.55 2.46 2.54 Cortex 6.66 6.34 5.29 4.39 5.35 5.62 6.35 6.72 30 min Caudal brain stem 7.51 7.90 8.48 6.59 6.78 6.71 6.36 6.79 Cerebellum 6.32 6.23 6.30 5.82 7.36 6.54 6.70 7.98 Striatum 7.60 6.59 5.35 5.17 4.66 4.75 4.64 3.53 Midbrain 7.70 7.52 6.75 5.97 3.67 3.39 2.93 2.99 Cortex 6.99 6.62 5.79 4.94 5.28 5.39 6.05 6.47 40 min Caudal brain stem 8.47 9.00 8.40 6.10 6.74 6.83 6.83 7.04 Cerebellum 6.07 5.97 5.69 5.19 6.00 6.05 6.70 7.44 Striatum 7.35 6.66 5.79 4.97 3.77 3.66 3.16 2.39 Midbrain 6.91 6.68 5.97 5.28 3.72 3.09 2.83 3.08 Cortex 7.87 7.18 6.00 4.95 5.16 5.46 5.85 5.73 ↵* Effect of smoothing was significant for 6- to 10-mo comparison, based on 2-way repeated-measures ANOVA with smoothing and scan length as factors.
