Article Figures & Data
Figures
- FIGURE 1.
Autoradiograms and corresponding immunohistochemical staining of insulin in pancreas of mouse treated with vehicle (A), mouse treated with 120 mg/kg of streptozotocin (B), and mouse treated with 200 mg/kg of streptozotocin (C) and injected with 99mTc-annexin A5. Boxes indicate magnified areas shown in insets. Black arrows = pancreatic islets (with positive insulin staining); red arrows = regions with high 99mTc-annexin A5 accumulation (with concomitant insulin staining). Red scale bars = 1,000 μm, and black scale bars (inset) = 300 μm. STZ = streptozotocin.
- FIGURE 2.
Autoradiograms and corresponding immunohistochemical staining of insulin in pancreas of NOD mice at 5 (A), 9 (B), 16 (C), and 20 wk of age (D) injected with 99mTc-annexin A5. Boxes indicate magnified areas shown in insets. Black arrows = pancreatic islets (positive to insulin staining); red arrows = regions with high 99mTc-annexin A5 accumulation (concordant with insulin staining). Red scale bars = 1,000 μm, and black scale bars (inset) = 300 μm.
- FIGURE 3.
TUNEL staining in pancreas of streptozotocin-treated mice and NOD mice injected with 99mTc-annexin A5. Arrows show TUNEL-positive cells. Boxes indicate magnified areas shown in insets. Blue scale bars = 100 μm, and black scale bars in inset = 25 μm. STZ = streptozotocin.
- FIGURE 4.
99mTc-annexin A5 accumulation level and number of TUNEL-positive cells in pancreatic islets of streptozotocin-treated mice and NOD mice injected with 99mTc-annexin A5. 99mTc-annexin A5 accumulation levels in pancreatic islets of streptozotocin-treated mice (A) and NOD mice (B) injected with 99mTc-annexin A5 and number of TUNEL-positive cells in pancreatic islets of streptozotocin-treated mice (C) and NOD mice (D) injected with 99mTc-annexin A5 are shown. Data represent mean ± SD (n = 5–8/group). STZ = streptozotocin.
- FIGURE 5.
Correlations between 99mTc-annexin A5 accumulation levels and number of TUNEL-positive cells in pancreatic islets of streptozotocin-treated mice (A) and NOD mice (B) injected with 99mTc-annexin A5. Data represent mean ± SD (n = 5–8/group).
Tables
Mouse group Body weight (g) Blood glucose level (mg/dL) Vehicle-treated (n = 5) 25.2 ± 1.0 103 ± 16 Streptozotocin-treated 120 mg/kg (n = 5) 23.8 ± 1.6 124 ± 37 200 mg/kg (n = 5) 25.7 ± 1.0 90 ± 30 NOD 5-wk-old (n = 8) 16.6 ± 1.1 119 ± 44 9-wk-old (n = 6) 20.6 ± 0.9* 132 ± 30 16-wk-old (n = 8) 24.1 ± 1.3*,† 119 ± 14 20-wk-old (n = 5) 22.2 ±1.3*,‡ 119 ± 30
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