Article Figures & Data
Figures
- FIGURE 1.
Coronal maximum-intensity-projection whole-body PET images after oral administration of 18F-FDG to conscious and anesthetized rats: 40 min (A) and 85 min (B).
- FIGURE 2.
Coronal maximum-intensity-projection abdominal PET images obtained serially after oral administration of 18F-FDG to conscious rat (A) and rats anesthetized with isoflurane (B) or propofol (C).
- FIGURE 3.
Time courses of radioactivity in stomach (A) and small intestine (B) after oral administration of 18F-FDG to conscious rats (○) and rats anesthetized with isoflurane (□) or propofol (▵). Data are mean ± SD of 3 experiments.
- FIGURE 4.
Compartment model for gastric emptying and intestinal absorption. D (X0) represents dose of 18F-FDG. Xs and Xi represent amount of 18F-FDG in stomach and intestine, respectively, at time t.
- FIGURE 5.
Time courses of radioactivity in whole blood after oral administration of 18F-FDG to conscious rats (○) and rats anesthetized with isoflurane (▵) or propofol (□). Data are mean ± SD of 3 experiments.
- FIGURE 6.
Intestinal permeability of d-glucose and 2-FDG in absence or presence of glucose transporter inhibitors. As inhibitor, phlorizin (1.0 mM, gray bar) or d-glucose (20 mM, black bar) was added in luminal solution with [UL-13C6]-d-glucose or 2-FDG (0.1 mM, white bar). Data are mean ± SD of 3 experiments. *P < 0.05 compared with respective inhibitor-free experiment by unpaired Student t test.
Tables
Gastric emptying Intestinal absorption State of rats kGE (min−1) T1/2 (min) kIT (min−1) T1/2 (min) Tmax (min) Conscious 0.217 ± 0.089 3.71 ± 1.89 0.057 ± 0.027 14.19 ± 6.79 6.96 ± 2.35 Anesthesia with isoflurane 0.014 ± 0.001* 50.32 ± 2.37* 0.033 ± 0.008 21.72 ± 4.86 41.83 ± 14.36 Anesthesia with propofol 0.022 ± 0.011* 36.46 ± 14.62* 0.034 ± 0.004 20.29 ± 2.48 18.17 ± 6.43 ↵* P < 0.005 compared with conscious rat group by ANOVA.
T1/2 = Half-life of radioactivity in stomach or intestine.
- TABLE 2.
Pharmacokinetic Parameters from Blood Profile after Oral Administration of 18F-FDG to Rats
State of rats Cmax (% of dose/mL) Tmax (min) Area under curve from 0 to 90 min (% of dose·min/mL) ka (min−1) Conscious 0.554 ± 0.045 23.3 ± 5.77 29.39 ± 2.32 0.076 ± 0.019 Anesthesia with isoflurane 0.327 ± 0.091* 50.0 ± 8.66* 20.29 ± 3.23* 0.016 ± 0.005* Anesthesia with propofol 0.410 ± 0.135 50.0 ± 8.66* 28.07 ± 7.66 0.020 ± 0.009* ↵* P < 0.005 compared with conscious rat group by ANOVA.
