Article Figures & Data
Figures
- FIGURE 1.
Image acquisition and registration scheme. Green double arrows represent rigid point-set registration used for autoradiography and Hoechst images from reference section. Blue dashed arrows represent manual rigid registration of microscopy images from nonreference sections. Red solid arrows correspond to Hoechst-based deformable registration.
- FIGURE 2.
Fragments of Hoechst images acquired from 2 consecutive sections showing corresponding landmark indicated with white cross.
- FIGURE 3.
Transparent overlay in false colors of 14C-FDG autoradiography (red), 18F-FLT autoradiography (green), and Hoechst (light blue). (A and C) Images manually registered on basis of observed tissue outline. (B and D) Same images after rigid point-set registration.
- FIGURE 4.
(A) Distribution of displacement values between corresponding landmarks after rigid point-set image registration (Hoechst image registered to 18F-FLT autoradiogram and 14C-FDG autoradiogram registered to 18F-FLT autoradiogram). (B) Distribution of displacement values between corresponding landmarks: before deformable image registration (red dotted line) and after deformable image registration (blue continuous line).
- FIGURE 5.
Transparent overlays of: (A and B) Hoechst images corresponding to section −1 (light blue) and section +1 (red); (C and D) pimonidazole from section −1 (green) and Hoechst from section +1 (red). Left panels present images before Hoechst-based registration, and right panels present same images after registration. Arrow indicates area highlighting how initial misalignment was corrected by deformable registration.
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