Article Figures & Data
Figures
- FIGURE 1.
In vitro evaluation of AdSSTR2, AdSSTR2-EGFP, and AdEGFP. (A) Representative saturation binding curves for 111In-DTPA-Y3-octreotate on membrane preparations from SCC-9 cells infected with AdSSTR2 or AdSSTR2-EGFP at 100 PFU per cell. Each data point represents mean ± SEM of triplicate measurements. (B) Fluorescence spectra of SCC-9 cells infected with AdEGFP or AdSSTR2-EGFP at 100 PFU per cell. norm = normalized.
- FIGURE 2.
Specific internalization of 111In-DTPA-Y3-octreotate at 37°C into SCC-9 cells infected with AdSSTR2 or AdSSTR2-EGFP at 100 PFU per cell. 111In-DTPA-Y3-octreotate (∼1.5 nM) was incubated with cells for various times in presence or absence of inhibitor. Cells were acid washed to remove surface-bound radioactivity and then harvested to determine internalized radioactivity. Data for each time point are presented as mean ± SEM of 3 experiments each performed in triplicate.
- FIGURE 3.
Biodistribution of 111In-DTPA-Y3-octreotate in mice bearing SCC-9 tumor xenografts. Tumors were injected directly with adenovirus vectors, and 111In-DTPA-Y3-octreotate was injected via tail vein 2 d later. Mice were sacrificed 4 h (A), 24 h (B), and 48 h (C) later (n = 5 for each group). Data are presented as % ID/g ± SEM.
- FIGURE 4.
Representative coronal nano-SPECT/CT images of SCC-9 tumor–bearing mice at 4, 24, and 48 h after injection of 111In-DTPA-Y3-octreotate. Axillary tumors were injected directly with Opti-MEM (control), AdSSTR2, or AdSSTR2-EGFP 2 d before administration of 111In-DTPA-Y3-octreotate. Coronal images show uptake of 111In-DTPA-Y3-octreotate in AdSSTR2- and AdSSTR2-EGFP-injected tumors but not control tumors (white arrows) and clearance through kidneys (yellow arrows).
- FIGURE 5.
Representative in vivo (A–C) and ex vivo (D–F) fluorescence imaging of mice bearing SCC-9 tumors injected directly with AdSSTR2 (A and D), AdSSTR2-EGFP (B and E), or AdEGFP (C and F) 2 d earlier. Tumor (arrow) was not visualized in vivo after injection of AdSSTR2 (A, negative control) but could be seen after injection of AdSSTR2-EGFP (B) or AdEGFP (C). In ex vivo studies, tumor had higher autofluorescence than liver and spleen (E), and fluorescence was observed in tumor injected with AdSSTR2-EGFP (D) or AdEGFP (F). au = arbitrary units.
