OtherBASIC SCIENCE INVESTIGATIONS

PET of Hypoxia with 89Zr-Labeled cG250-F(ab′)2 in Head and Neck Tumors

Bianca A.W. Hoeben, Johannes H.A.M. Kaanders, Gerben M. Franssen, Esther G.C. Troost, Paul F.J.W. Rijken, Egbert Oosterwijk, Guus A.M.S. van Dongen, Wim J.G. Oyen, Otto C. Boerman and Johan Bussink
Journal of Nuclear Medicine July 2010, 51 (7) 1076-1083; DOI: https://doi.org/10.2967/jnumed.109.073189

Article Figures & Data

Figures

  • FIGURE 1. 
    FIGURE 1. 

    Gray-scale images of tumor sections immunohistochemically stained for CAIX (A and C) and autoradiograms of 111In-cG250 (B and D) at 4 (A and B) and 24 h (C and D) after injection. At 4 h after injection, uptake of 111In-cG250 is nonspecific and not localized in region of high CAIX expression. At 24 h after injection, more distinct correlation is present. ARG = autoradiography; IHC = immunohistochemistry; p.i. = after injection.

  • FIGURE 2. 
    FIGURE 2. 

    (A) 89Zr-cG250-F(ab′)2 autoradiogram. (B and C) Image of tumor section immunohistochemically stained for CAIX (red) and vessels (green) (B) and pimonidazole (green) and CAIX (red) (C). (D) Scatterplot between spatial intensity patterns of 89Zr-cG250-F(ab′)2 and CAIX in this SCCNij3 tumor section at 4 h after injection. Each dot in D represents 200 × 200 μm coregistered pixel. ARG = autoradiography; IHC = immunohistochemistry; p.i. = after injection.

  • FIGURE 3. 
    FIGURE 3. 

    Disparity between vessels (green) and localization of high 89Zr-cG250-F(ab′)2 signal (red) in tumor sections at 4 (A) and 24 h (B) after injection. As early as 4 h after injection, 89Zr-cG250-F(ab′)2 binding in tumor was not perfusion-dependent. Central vessel in B (arrow) was not perfused (absence of Hoechst 33342 staining; for clarity Hoechst signal is not shown). p.i. = after injection.

  • FIGURE 4. 
    FIGURE 4. 

    PET images of mice with tumor located subcutaneously on right hind leg at 4 (A) and 24 h (B) after injection. p.i. = after injection; SUV = standardized uptake value.

  • FIGURE 5. 
    FIGURE 5. 

    Biodistribution of 89Zr-cG250-F(ab′)2 in tumor, blood, and muscle at 4 and 24 h after injection (A). Tumor uptake of 89Zr-cG250-F(ab′)2 in biodistribution measurements vs. SUVmax of tumor on PET scan (B). p.i. = after injection.

Tables

  • TABLE 1

    Spatial Correlation Between 89Zr-cG250-F(ab′)2 Autoradiography and Immunohistochemical Signal of CAIX and Pimonidazole

    89Zr-cG250-F(ab′)2 vs. CAIX89Zr-cG250-F(ab′)2 vs. pimonidazole
    Time after injection of 89Zr-cG250-F(ab′)2 (h)Mean correlation coefficient (r)Mean slope (α)Mean correlation coefficient (r)Mean slope (α)
    4 (n = 4)0.68 ± 0.060.31 ± 0.150.54 ± 0.080.25 ± 0.07
    24 (n = 4)0.62 ± 0.040.36 ± 0.280.61 ± 0.090.38 ± 0.11
    All (n = 8)0.65 ± 0.050.33 ± 0.210.58 ± 0.090.31 ± 0.11

    • Data are given as mean ± SD. Correlation coefficients (r) and mean linear regression slopes (α) result from 200 × 200 μm pixel-by-pixel comparison between 89Zr-cG250-F(ab′)2 autoradiography images and immunohistochemical signal images of CAIX and pimonidazole.

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