Article Figures & Data
Figures
- FIGURE 1.
Photographs of tumor-bearing mouse before PDT (A), 1 d after PDT (B), and 1 mo after PDT (C). CWR22 tumor (arrow) showed rapid response 1 d after treatment but had disappeared 1 mo after therapy.
- FIGURE 2.
Transverse small-animal PET images of 2 PC-3 tumors (arrows). Images of treated tumor before PDT (A) and 48 h after PDT (C) show that 11C-choline uptake of treated tumor had decreased 48 h after PDT. Images of control tumor before PDT (B) and 48 h after PDT (D) show slightly increased 11C-choline uptake 48 h after PDT. Quantitative analysis results of 11C-choline uptake in treated and control tumors confirmed our visual inspection; these results are shown in Figure 3.
- FIGURE 3.
Time–activity curves of 11C-choline in PDT-treated PC-3 tumors (n = 4) (A) and in control tumors (n = 4) (B) of same mice before PDT and 48 h after PDT. Uptake of 11C-choline was measured as %ID/g. Error bars represent SEs.
- FIGURE 4.
Histologic images of treated and control PC-3 tumors at 48 h after PDT. Inflammatory response with edema was observed in treated tumor (A) but was not seen in control tumor (B). Rectangular areas on A and B are magnified and shown in C and D, respectively. In C, massive areas of treated tissue were damaged by PDT; however, control tumor cells remained intact (D).
- FIGURE 5.
Pc 4-PDT–induced changes in 11C-choline uptake as function of post-PDT incubation time (5, 30, and 45 min). Activity of 11C-choline in PC-3 cells was measured by γ-counter, and unit was counted per minute (CPM). Each data point represents 3 wells of cells, and error bars are SD.
Tables
- TABLE 1
PSA Values (ng/mL) of Treated Mice and 11C-Choline Uptake of Treated Tumors (CWR22) Before PDT and 48 Hours After PDT
Mouse ID Parameter M1 M2 PSA Before 44.6 66.6 24 h 34.8 41.8 48 h 28.1 29.3 %ID/g Before 12.5 ± 1.5 19.0 ± 1.9 48 h 9.5 ± 1.8 7.8 ± 1.3 %ID/g response −24.1% −59.2% Uptake was measured by %ID/g. Numbers are means ± SDs of measurements at 7 frames (5, 15, 25, 35, 45, 53, and 57 min).
- TABLE 2
11C-Choline Uptake of PDT-Treated and Control Tumors (PC-3) Before PDT and 48 Hours After PDT
Mouse ID Parameter M3 M4 M5 M6 Treated tumors Before 10.2 ± 0.9 21.2 ± 1.8 22.2 ± 1.6 17.3 ± 2.6 48 h 6.4 ± 0.4 12.2 ± 2.3 13.0 ± 1.6 12.1 ± 1.5 %ID/g response −37.3% −42.5% −41.5% −30.0% Control tumors Before 12.9 ± 0.5 22.5 ± 1.3 23.1 ± 3.2 19.5 ± 1.7 48 h 17.0 ± 0.4 27.5 ± 2.5 34.4 ± 3.6 26.3 ± 2.2 %ID/g response 32.3% 22.3% 48.8% 34.9% Numbers are %ID/g. Means ± SDs of %ID/g were calculated at 7 frames (5, 15, 25, 35, 45, 53, and 57 min). Each mouse (M3–M6) had 2 tumors; one was treated and other served as control.
- TABLE 3
PSA Values (ng/mL) of PDT-Treated Mice and 11C-Choline Uptake of Treated Tumors (CWR22) Before PDT and 24 Hours After PDT
Mouse ID Parameter M7 M8 M9 M10 M11 PSA Before 27.4 48.4 20.4 43.6 NA 24 h 26.4 34.5 14.3 35.5 NA %ID/g Before 16.2 ± 3.6 14.2 ± 2.4 15.1 ± 3.8 17.7 ± 1.7 23.6 ± 2.3 24 h 1.0 ± 0.2 4.6 ± 0.3 7.2 ± 1.1 4.2 ± 0.8 4.2 ± 1.0 %ID/g response −94.1% −67.3% −52.2% −76.0% −82.0% Uptake was measured as %ID/g. Numbers are means ± SDs at 7 frames (5, 15, 25, 35, 45, 53, and 57 min). PSA was not available (NA) for mouse M11.
- TABLE 4
11C-Choline Uptake of Treated and Control Tumors (PC-3) Before PDT and 1 Hour After PDT
Mouse ID Parameter M12 M13 M14 M15 Treated tumors Before 11.9 ± 1.3 13.6 ± 1.9 24.9 ± 2.9 23.3 ± 3.8 1 h 11.0 ± 0.7 8.5 ± 1.2 14.7 ± 3.0 17.6 ± 3.0 %ID/g response −8.1% −37.3% −41.0% −24.4% Control tumors Before 14.7 ± 1.9 19.4 ± 2.7 21.2 ± 2.9 27.4 ± 5.3 1 h 16.4 ± 1.0 23.9 ± 1.9 26.6 ± 1.3 23.7 ± 1.2 %ID/g response 11.6% 23.2% 25.8% −13.3% Numbers represent %ID/g and %ID/g response. Means ± SDs were calculated from 7 frames (5, 15, 25, 35, 45, 53, and 57 min).
Time after PDT (min) Dish no. Total Viable Nonviable Nonviable % 30 1 200 193 7 3.5% 2 200 198 2 1.0% 3 200 196 4 2.0% 45 4 224 217 7 3.1% 5 235 225 10 4.3% 6 452 430 22 4.9% 60 7 208 203 5 2.4% 8 250 244 6 2.4% 9 273 268 5 1.8% 450 10 227 210 17 7.5% 11 230 212 18 7.8% 12 213 193 20 9.4% No PDT450 13 211 207 4 1.9% 14 322 315 7 2.2% 15 203 198 5 2.5% Cell cultures were stained with trypan blue solution. Unstained (viable) and stained (nonviable) cells were counted for each dish (nos. 1–15). Percentages of nonviable cells were calculated for both treated and control dishes.
