Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Corporate & Special Sales
    • Journal Claims
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Permissions
    • Advertisers
    • Continuing Education
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Corporate & Special Sales
    • Journal Claims
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Permissions
    • Advertisers
    • Continuing Education
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • Follow JNM on Twitter
  • Visit JNM on Facebook
  • Join JNM on LinkedIn
  • Subscribe to our RSS feeds
Meeting ReportOncology - Basic: Basic Science

Validation of 18F-fluorothymidine (FLT) as in vivo molecular probe for measurement of tumor DNA repair synthesis

Malik Juweid, Andreas Buck and Janina Baranowska-Kotylewicz
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 636;
Malik Juweid
1University of Iowa, Iowa City, IA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andreas Buck
2Technical University of Munich, Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Janina Baranowska-Kotylewicz
3University of Nebraska, Omaha, NE
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

636

Objectives FLT has been validated as in vivo marker of tumor cell proliferation. We validated herein its use for a novel application: measurement of tumor DNA repair synthesis.

Methods To measure DNA repair synthesis unconfounded by proliferation-related replicative DNA synthesis, hydroxyurea (HU), known to both inhibit cell proliferation and trigger DNA repair, was used. Cultured actively growing A549 lung adenocarcinoma cells were continuously exposed to 0 or 0.5 mM HU for 72h, a concentration shown to arrest tumor cell growth between 48 and 72h post HU exposure. HU-containing media was removed, cells rinsed and media containing ~10 µCi/ml of 18FLT added for 60 min. Cells were washed, harvested and cellular FLT uptake, cell number, viability and cell-cycle distribution determined.

Results Cell viability was similar in the HU-exposed and control cells (80% vs. 88%). FLT uptake per A549 cell at 72h post HU exposure was higher than that of control cells (0.0025 vs. 0.0016 cpm/cell) despite only a 1.4-fold increase in cell number during the 72-h incubation period vs. a 2.8-fold increase in control cells. %HU-exposed cells in S-phase of cell cycle was ~3x higher than in control cells (63% vs. 22%) indicating intra-S cycle arrest of HU-exposed cells and that increased cellular FLT uptake is due to intra-S DNA repair synthesis.

Conclusions Our data validate use of FLT for measuring DNA repair in vitro and in vivo after treatment with chemotherapeutics known to induce cell cycle arrest with cessation of replicative DNA synthesis (e.g., HU, camptothecin, gemcitabine). This approach could allow assessment of resistance to drugs caused by enhanced tumor DNA repair.

  • © 2009 by Society of Nuclear Medicine
Back to top

In this issue

Journal of Nuclear Medicine
Vol. 50, Issue supplement 2
May 2009
  • Table of Contents
  • Index by author
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Validation of 18F-fluorothymidine (FLT) as in vivo molecular probe for measurement of tumor DNA repair synthesis
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
Validation of 18F-fluorothymidine (FLT) as in vivo molecular probe for measurement of tumor DNA repair synthesis
Malik Juweid, Andreas Buck, Janina Baranowska-Kotylewicz
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 636;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Validation of 18F-fluorothymidine (FLT) as in vivo molecular probe for measurement of tumor DNA repair synthesis
Malik Juweid, Andreas Buck, Janina Baranowska-Kotylewicz
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 636;
Twitter logo Facebook logo LinkedIn logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
  • Info & Metrics

Related Articles

  • No related articles found.
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

Oncology - Basic: Basic Science

  • Radiolabeled inhibitors of seprase targeting the tumor microenvironment
  • Transcriptome foundations of low FDG uptake in prostate cancer
  • The change of F-18 FDG and H-3 PCV uptakes after transduction of micro RNA for hexokinase II and HSV1-sr39 gene in cancer cells
Show more Oncology - Basic: Basic Science

Biomarkers and Radiation Effects

  • Analysis of DNA double-strand breaks in gastric cancer cells after treatment with 213Bi-immunoconjugates
  • In vivo imaging of long term stem cell trafficking/engraftment is enabled by a human somatostatin receptor type-2 (hSSTR2) mutant that has the desirable features of being muted in altering stem cell signaling and function
Show more Biomarkers and Radiation Effects

Similar Articles

SNMMI

© 2023 Journal of Nuclear Medicine

Powered by HighWire