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Meeting ReportRadiopharmaceutical Chemistry: New Radiopharmaceuticals

Evaluation of [64Cu]DOTA-annexin V radiotracer in the cycloheximide-induced liver apoptosis model

Xiangjun Meng, Paul McQuade, Marie-Jose Belanger, Amy Vanko, Brett Connolly, Karen Schlingmann, Caroline Houde, Huseyin Mehmet, Ilonka Guenther and Jacquelynn Cook
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 1950;
Xiangjun Meng
1Merck Research Laboratories, Imaging, West Point, PA
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Paul McQuade
1Merck Research Laboratories, Imaging, West Point, PA
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Marie-Jose Belanger
1Merck Research Laboratories, Imaging, West Point, PA
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Amy Vanko
1Merck Research Laboratories, Imaging, West Point, PA
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Brett Connolly
1Merck Research Laboratories, Imaging, West Point, PA
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Karen Schlingmann
1Merck Research Laboratories, Imaging, West Point, PA
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Caroline Houde
2Merck Research Laboratories, Apoptosis, Rahway, NJ
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Huseyin Mehmet
2Merck Research Laboratories, Apoptosis, Rahway, NJ
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Ilonka Guenther
1Merck Research Laboratories, Imaging, West Point, PA
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Jacquelynn Cook
1Merck Research Laboratories, Imaging, West Point, PA
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Abstract

1950

Objectives This study was designed to evaluate [64Cu] labeled annexin V as a PET tracer in a rat model of cycloheximide (CHX) induced liver apoptosis.

Methods A pilot study showed that a single IV injection of CHX rapidly induced liver apoptosis in rat dose-dependently; maximum apoptosis in the liver occurred at 3 h after CHX treatment. In this study, male Sprague Dawley (SD) rats were treated IV with 5 or 10mg/kg CHX to induce liver apoptosis. 3 h after the treatment, rats were imaged for 90min with 0.5-1.0 µCi/g [64Cu] labeled annexin V. The livers were harvested at the end of the scan for ex vivo autoradiography and terminal deoxynucleotide end-labeling (TUNEL) staining.

Results Annexin V tracer uptake in the liver increased dose dependently with CHX treatment. Liver uptake ratios (treated/control) were 2.87 and 3.39 for 5 and 10 mg/kg respectively. Kidney uptake decreased with increased CHX dose. There were no differences in the blood pool activity between treated and control rats. Ex vivo autoradiography and TUNEL staining showed good correlation of annexin V uptake with TUNEL-positive cells. There was a positive correlation between liver annexin V standardized uptake values at the end of the PET scan and numbers of the TUNEL-positive cells.

Conclusions PET studies combined with ex vivo autoradiography and TUNEL staining showed that uptake of [64Cu] labeled annexin V correlates well with the level of apoptotic cells in this CHX induced liver apoptosis model.

  • © 2009 by Society of Nuclear Medicine
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Journal of Nuclear Medicine
Vol. 50, Issue supplement 2
May 2009
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Evaluation of [64Cu]DOTA-annexin V radiotracer in the cycloheximide-induced liver apoptosis model
Xiangjun Meng, Paul McQuade, Marie-Jose Belanger, Amy Vanko, Brett Connolly, Karen Schlingmann, Caroline Houde, Huseyin Mehmet, Ilonka Guenther, Jacquelynn Cook
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 1950;

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Evaluation of [64Cu]DOTA-annexin V radiotracer in the cycloheximide-induced liver apoptosis model
Xiangjun Meng, Paul McQuade, Marie-Jose Belanger, Amy Vanko, Brett Connolly, Karen Schlingmann, Caroline Houde, Huseyin Mehmet, Ilonka Guenther, Jacquelynn Cook
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 1950;
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