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Meeting ReportOncology - Basic: Basic Science

Hypoxia imaging agent 64Cu-ATSM visualizes cancer stem cell-rich regions within tumors

Yukie Yoshii, Yasushi Kiyono, Atsuo Waki, Tetsuya Mori, Hiroshi Yoshii, Takako Furukawa, Myungmi Oh, Masato Kobayashi, Michael J. Welch and Yasuhisa Fujibayashi
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 1554;
Yukie Yoshii
1University of Fukui, BIRC, Fukui, Japan
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Yasushi Kiyono
1University of Fukui, BIRC, Fukui, Japan
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Atsuo Waki
1University of Fukui, BIRC, Fukui, Japan
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Tetsuya Mori
1University of Fukui, BIRC, Fukui, Japan
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Hiroshi Yoshii
1University of Fukui, BIRC, Fukui, Japan
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Takako Furukawa
2National Institute of Radiological Sciences, MIC, Chiba, Japan
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Myungmi Oh
1University of Fukui, BIRC, Fukui, Japan
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Masato Kobayashi
1University of Fukui, BIRC, Fukui, Japan
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Michael J. Welch
3Washington University, MIR, St. Louis, MO
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Yasuhisa Fujibayashi
1University of Fukui, BIRC, Fukui, Japan
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Abstract

1554

Objectives 64Cu-diacetyl-bis (N4-methylthiosemicarbazone) (64Cu-ATSM) is a PET imaging agent targeting tumor hypoxia. It is known that tumor hypoxia assessed by Cu-ATSM is associated with tumor malignant behavior, such as resistance to therapy and metastasis ability. Recent studies demonstrated that the tumor characteristics of therapeutic resistance and metastasis ability would be attributed to so-called cancer stem cells. Based on these understandings, we hypothesized that tumor hypoxic regions visualized by 64Cu-ATSM would contain higher proportions of cancer stem cells than other tumor regions. In this study, we examined distribution of cancer stem cells within tumors and compared it with accumulations of 64Cu-ATSM and 18FDG.

Methods Double-tracer autoradiography and immunohistchemistry was performed with mouse colon carcinoma (Colon-26) tumor-bearing mice. In autoradiography, mixture of 74 MBq of 18FDG and 0.37 MBq of 64Cu-ATSM was i.v. injected. CD133 was used as a cancer stem cell marker, which reflected typical characteristics of cancer stem cells in Colon-26 cells.

Results Percentage of CD133+ cells was high in order of 64Cu-ATSM high uptake regions >64Cu-ATSM middle uptake regions > 64Cu-ATSM low uptake regions > 18FDG high uptake regions within Colon-26 tumors. The density of CD133+ cells was 11-fold higher in 64Cu-ATSM high uptake regions than in 18FDG high uptake regions.

Conclusions 64Cu-ATSM accumulating hypoxic regions matched CD133+ cell-rich regions within Colon-26 tumors. Our findings suggested that 64Cu-ATSM is a potential imaging agent for cancer stem cell-rich regions within tumors.

Research Support R.W. helps 64Cu-ATSM synthesis.

  • © 2009 by Society of Nuclear Medicine
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Journal of Nuclear Medicine
Vol. 50, Issue supplement 2
May 2009
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Hypoxia imaging agent 64Cu-ATSM visualizes cancer stem cell-rich regions within tumors
Yukie Yoshii, Yasushi Kiyono, Atsuo Waki, Tetsuya Mori, Hiroshi Yoshii, Takako Furukawa, Myungmi Oh, Masato Kobayashi, Michael J. Welch, Yasuhisa Fujibayashi
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 1554;

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Hypoxia imaging agent 64Cu-ATSM visualizes cancer stem cell-rich regions within tumors
Yukie Yoshii, Yasushi Kiyono, Atsuo Waki, Tetsuya Mori, Hiroshi Yoshii, Takako Furukawa, Myungmi Oh, Masato Kobayashi, Michael J. Welch, Yasuhisa Fujibayashi
Journal of Nuclear Medicine May 2009, 50 (supplement 2) 1554;
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