Simultaneous X-Ray and near infrared fluorescence imaging of infiltrating cells in small animals

Abstract

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Objectives: The purpose of this study is to determine if fluorescence (Near Infrared) and X-Ray contrast agent laden cells can be used for simultaneous multi-modal imaging of cells in mice to access cell sequestration or infiltration during inflammation.

Methods: Murine cells in culture were laden with KODAK X-SIGHT nanospheres and X-Ray contrast nanoparticles, and injected in athymic mice intravenously under anesthesia. A KODAK In-Vivo Imaging System FX that combines high-sensitivity optical imaging and high-resolution digital X-Ray imaging was used to obtain anatomical localization of the exogenous murine cells in the athymic mice at different time intervals.

Results: The optical and X-Ray contrast agents signals co localize in vivo along with the exogenous murine cells. Simultaneously obtained X-Ray and near infrared fluorescence (NIRF) images of the exogenous murine cells, coregisters signal localization on anatomical overlays.

Conclusions: Murine cells in culture can be laden with fluorescent and X-Ray contrast nanoparticles simultaneously and successfully imaged by optical and X-Ray modalities with precise co registration of bimodal signal in both in vitro and in vivo applications. The benefits of the combined high-resolution X-Ray and high sensitivity NIRF imaging properties packaged together will aid diagnostic modules in atherosclerotic plaque, cancer, and inflammatory pathways where infiltration of macrophages occur.