PT  - JOURNAL ARTICLE
AU  - Papineni, Rao
AU  - McLaughlin, William
AU  - Vizard, Douglas
AU  - Leevy, W.Matthew
AU  - Feke, Gilbert
AU  - Manisha, Patel
TI  - Simultaneous X-Ray and near infrared fluorescence imaging of infiltrating cells in small animals
DP  - 2008 May 01
TA  - Journal of Nuclear Medicine
PG  - 161P--161P
VI  - 49
IP  - supplement 1
4099  - http://jnm.snmjournals.org/content/49/supplement_1/161P.2.short
4100  - http://jnm.snmjournals.org/content/49/supplement_1/161P.2.full
SO  - J Nucl Med2008 May 01; 49
AB  - 702 Objectives: The purpose of this study is to determine if fluorescence (Near Infrared) and X-Ray contrast agent laden cells can be used for simultaneous multi-modal imaging of cells in mice to access cell sequestration or infiltration during inflammation. Methods: Murine cells in culture were laden with KODAK X-SIGHT nanospheres and X-Ray contrast nanoparticles, and injected in athymic mice intravenously under anesthesia. A KODAK In-Vivo Imaging System FX that combines high-sensitivity optical imaging and high-resolution digital X-Ray imaging was used to obtain anatomical localization of the exogenous murine cells in the athymic mice at different time intervals. Results: The optical and X-Ray contrast agents signals co localize in vivo along with the exogenous murine cells. Simultaneously obtained X-Ray and near infrared fluorescence (NIRF) images of the exogenous murine cells, coregisters signal localization on anatomical overlays. Conclusions: Murine cells in culture can be laden with fluorescent and X-Ray contrast nanoparticles simultaneously and successfully imaged by optical and X-Ray modalities with precise co registration of bimodal signal in both in vitro and in vivo applications. The benefits of the combined high-resolution X-Ray and high sensitivity NIRF imaging properties packaged together will aid diagnostic modules in atherosclerotic plaque, cancer, and inflammatory pathways where infiltration of macrophages occur.