Article Figures & Data
Figures
- FIGURE 1.
(A) Diagram illustrating primary structure of duramycin with cross-linking bridges. (B and C) Radio-HPLC chromatograms of 99mTc-duramycin before and after purification are shown in B and C, respectively. Low, but significant, level of 99mTc-pertechnetate is present before purification with retention time of 2 min. (D) Representative competition curve of 3 independent experiments. 99mTc-duramycin binding of apoptotic cells is competitively diminished by presence of PtdE-containing liposomes (•) but not other phospholipid species, including PtdC (▪), PtdG (□), or PtdS (○). cpm = counts per minute.
- FIGURE 2.
(A) Blood clearance of 99mTc-duramycin in healthy rats (n = 3). (B–E) Radio-HPLC chromatograms of 99mTc-duramycin standard (B), serum at 1 min after injection (C), serum at 5 min after injection (D), and urine at 60 min after injection (E).
- FIGURE 3.
(A) Dynamic planar imaging of 99mTc-duramycin distribution in healthy rat. Note rapid renal clearance of radiotracer and low hepatic uptake. (B) Whole-body dynamic planar imaging of 99mTc-duramycin uptake in rat with acute myocardial infarction. (C) Non–color-enhanced, raw counts of static planar images of sham-operated rat (left) and infarcted rat (right) acquired at 120 min after intravenous injection of 99mTc-duramycin. Infarct site is marked by arrows. In autoradiography, radioactivity uptake in myocardium colocalizes with infarct with excellent infarct-to-noninfarct ratio (inset). B = bladder; K = kidney.
Tables
Organ/tissue Uptake (%ID/g) at 60 min after injection Brain 0.01 ± 0.01 Thyroid 0.08 ± 0.03 Lung 0.12 ± 0.05 Heart 0.21 ± 0.16 Liver 0.28 ± 0.01 Pancreas 0.05 ± 0.01 Spleen 0.10 ± 0.02 Kidney 2.32 ± 0.48 Stomach 0.11 ± 0.01 Small intestine 0.37 ± 0.07 Colon 0.11 ± 0.01 Bone 0.04 ± 0.01 Muscle 0.03 ± 0.01 Fat 0.06 ± 0.06 Skin 0.01 ± 0.01 Blood 0.33 ± 0.11 Thymus 0.06 ± 0.02 Urine 22.1 ± 13.25
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