OtherBASIC SCIENCE INVESTIGATIONS

Time Course of Alterations in Myocardial Glucose Utilization in the Zucker Diabetic Fatty Rat with Correlation to Gene Expression of Glucose Transporters: A Small-Animal PET Investigation

Kooresh I. Shoghi, Robert J. Gropler, Terry Sharp, Pilar Herrero, Nicole Fettig, Yi Su, Mayurranjan S. Mitra, Attila Kovacs, Brian N. Finck and Michael J. Welch
Journal of Nuclear Medicine August 2008, 49 (8) 1320-1327; DOI: https://doi.org/10.2967/jnumed.108.051672

Article Figures & Data

Figures

  • FIGURE 1. 
    FIGURE 1. 

    Peripheral insulin (A) and glucose (B) levels at W14 and W19 in lean and ZDF rats. Significance values are denoted above bar plots. P value of less than 0.05 was considered significant.

  • FIGURE 2. 
    FIGURE 2. 

    MGUp (A) and MGU (B) at W14 and W19 in lean and ZDF rats. Significance values are denoted above bar plots. P value of less than 0.05 was considered significant.

  • FIGURE 3. 
    FIGURE 3. 

    (A) GLUT-1 and GLUT-4 gene expression levels in lean and ZDF rats at W19. Gene expression was normalized to levels observed in lean rats. Significance values are denoted above bar plots. P value of less than 0.05 was considered significant. (B) Representative autoradiographic results of Western blot analyses performed with antibodies for GLUT-1 and GLUT-4 on whole-cell lysates of hearts of lean and ZDF rats at W19. AU = arbitrary units.

  • FIGURE 4. 
    FIGURE 4. 

    Regression model M3 was used to characterize dependence of MGUp on gene expression of GLUT-4 with R2 = 0.81, which is significant at P = 0.0003.

Tables

  • TABLE 1

    Animal Demographics, Hemodynamics, and FFA Levels for Lean and ZDF Rats

    LeanZDF
    DataW14W19W14W19
    Weight (g)306.37 ± 11.19354.65 ± 23.16316.35 ± 17.66344.08 ± 21.21
    HR (bpm)245.50 ± 21.20*249.33 ± 31.39*207.17 ± 13.29198.17 ± 14.61
    HbA1C (%)3.33 ± 0.12*3.28 ± 0.10*7.72 ± 0.667.38 ± 0.73
    FFA (nmol/mL)825.90 ± 300.50*1165.32 ± 374.221728.72 ± 675.591920.00 ± 890.99

    • ↵* Significantly lower than age-matched ZDF rats.

    • Values are represented as mean ± 1 SD. P < 0.05 was considered significant. Please refer to “Results” for significance values. HR = heart rate; bpm = beats per minute.

  • TABLE 2

    ECHO Measurements for Lean and ZDF Rats

    LeanZDF
    ECHO measurementsW13W18W13W18
    Distolic LVPW (mm)1.44 ± 0.051.52 ± 0.051.43 ± 0.071.46 ± 0.09
    Systolic LVPW (mm)2.68 ± 0.152.58 ± 0.322.58 ± 0.092.61 ± 0.14
    Diastolic LVID (mm)8.56 ± 0.28*†8.96 ± 0.279.16 ± 0.329.22 ± 0.57
    Systolic LVID (mm)5.13 ± 0.555.86 ± 1.055.53 ± 0.295.42 ± 0.39
    LVM (mg)903.39 ± 38.45*†1054.53 ± 42.18996.14 ± 70.241044.00 ± 98.85
    FS (%)40.12 ± 4.6534.82 ± 10.2139.72 ± 1.7241.18 ± 3.08
    Rm-18F0.75 ± 0.010.76 ± 0.020.74 ± 0.020.75 ± 0.02

    • ↵* Significantly different from age-matched ZDF rats.

    • ↵† Significantly different from week 18.

    • Values are represented as mean ± 1 SD. P < 0.05 was considered significant. Please refer to “Results” for significance values. Rm-18F = myocardial recovery coefficient of 18F radionuclide.

  • TABLE 3

    Statistical Analysis of Regression Models Depicting Dependence of MGUp on Gene Expression of GLUT-1 and GLUT-4

    Significance of coefficientsANOVA
    Modelβ0β1β2F valueP valueR2
    M10.52300.72800.002014.70900.00500.83
    M20.35600.39400.82600.39400.11
    M30.29700.000338.20000.00030.81
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