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Figures
- FIGURE 1.
99mTc-radiolabeling of SDF-1α. Solid-phase prelabeled [99mTc-MAS3]-NHS was conjugated covalently to recombinant SDF-1α in DMSO in presence of base, releasing only NHS as byproduct. Primary amines available for conjugation are indicated by * in peptide sequence.
- FIGURE 2.
Purification and stability of [99mTc-MAS3]-SDF-1α. Purification of [99mTc-MAS3]-SDF-1α using GFC and simultaneous monitoring of 280-nm absorbance (A) and γ-ray activity (B). Retention times of major peaks are also shown. Tracings are representative of 8 independent labeling reactions.
- FIGURE 3.
In vivo biodistribution and clearance of [99mTc-MAS3]-SDF-1α. (A) Blood activity curves (mean ± SD) of 99mTc-MAS3 alone. (B) [99mTc-MAS3]-SDF-1α after intravenous injection of 37 MBq (1 mCi) into sham-operated rats. Also shown are calculated half-lives. Results are from 3 independent experiments. (C) Total organ distribution (%ID/g; mean ± SD) in the rats from Figure 3A at 2 h after injection.
- FIGURE 4.
Quantitation of functional CXCR4 expression in myocardial infarction. Uptake (%ID/g; mean ± SEM) of [99mTc-MAS3]-SDF-1α (A) and 99mTc-labeled albumin (B) 24 h after sham operation (Sham) or ischemia reperfusion–induced myocardial infarction and 2 h after intravenous injection of 37 MBq (1 mCi) of radiotracer. Normal myocardium and infarcted myocardium from MI hearts were quantitated separately. Results are from 4 rats per group. (C) Confocal fluorescence microscopy of hearts from Figure 4A stained with mouse anti-SDF-1α antibody (green) and myocyte probe phalloidin (red). Nuclei are counterstained with DAPI (blue). (D) Confocal fluorescence microscopy of hearts from Figure 4A stained with anti-CXCR4 antibody (green) and anti-SDF-1α antibody (red). Nuclei are counterstained with DAPI (blue).
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