Article Figures & Data
Figures
- FIGURE 1.
Representative saturation binding assays for different 6-OHDA doses. Specific binding data are means ± SEM of triplicate determinations at each 3H-mazindol concentration. Solid lines are nonlinear regression fits of data to a 1-site binding model. For each assay, estimated values of Bmax and KD, and the associated uncertainties in those estimates, are shown.
- FIGURE 2.
(A) Effect of 6-OHDA–induced denervation on cardiac NET density (Bmax). (B) Effect of 6-OHDA–induced denervation on cardiac 11C-HED uptake at 30 min. (C) Dependence of cardiac 11C-HED uptake at 30 min on NET density (Bmax).
- FIGURE 3.
Proposed comprehensive compartmental model of cardiac HED kinetics. Arrow thicknesses indicate relative magnitudes of the rate constants. Cp = concentration in plasma; CE = concentration in extracellular space; Caxo = concentration in neuronal axoplasm; Cves = concentration in vesicles.
- FIGURE 4.
Expected nonlinear relationship between HED retention and regional sympathetic nerve density in human heart, based on the comprehensive compartmental model shown in Figure 3.
Tables
- TABLE 1
Averages of NET Bmax, 3H-Mazindol Binding Affinity (KD), and HED Tissue Concentrations
6-OHDA dose (mg/kg) n NET Bmax (fmol/mg protein) KD (nmol/L) HED concentration at 30 min (%ID/g) Whole heart Left atrium Right atrium Left ventricle Right ventricle Blood 0 5 388 ± 23 0.72 ± 0.07 2.89 ± 0.35 2.95 ± 0.36 2.60 ± 0.27 2.73 ± 0.34 3.51 ± 0.46 0.073 ± 0.006 7 5 266 ± 45 0.73 ± 0.14 2.14 ± 0.33 2.01 ± 0.52 2.25 ± 0.64 1.96 ± 0.34 2.73 ± 0.34 0.081 ± 0.011 11 5 167 ± 94 1.08 ± 0.32 1.18 ± 0.87 0.74 ± 0.46 1.35 ± 0.84 1.09 ± 0.80 1.54 ± 1.26 0.105 ± 0.013 15 5 181 ± 65 0.94 ± 0.24 1.33 ± 0.56 0.93 ± 0.76 1.34 ± 0.48 1.26 ± 0.56 1.65 ± 0.59 0.105 ± 0.011 22 5 100 ± 31 0.95 ± 0.48 0.76 ± 0.32 0.32 ± 0.09 0.56 ± 0.29 0.75 ± 0.32 0.90 ± 0.38 0.102 ± 0.009 100 5 31 ± 6 1.83 ± 0.91 0.22 ± 0.06 0.18 ± 0.05 0.22 ± 0.05 0.22 ± 0.06 0.22 ± 0.05 0.091 ± 0.023 Values are mean ± SD.
- TABLE 2
Averages of NET Bmax, KD, and HED Concentrations for Hearts with Comparable NET Densities
NET Bmax range (fmol/mg protein) n NET Bmax (fmol/mg protein) KD (nmol/L) HED concentration at 30 min (%ID/g) Whole heart Left atrium Right atrium Left ventricle Right ventricle Blood ≥350 5 388 ± 23 0.72 ± 0.07 2.89 ± 0.35 2.95 ± 0.36 2.60 ± 0.27 2.73 ± 0.34 3.51 ± 0.46 0.073 ± 0.006 300–349 2 320 ± 1 0.70 ± 0.11 2.59 ± 0.05 2.04 ± 1.00 2.53 ± 0.26 2.42 ± 0.08 3.28 ± 0.33 0.081 ± 0.002 250–299 4 276 ± 16 0.74 ± 0.13 2.06 ± 0.18 2.03 ± 0.31 2.33 ± 0.36 1.89 ± 0.19 2.58 ± 0.37 0.082 ± 0.013 200–249 0 — — — — — — — — 150–199 5 177 ± 13 0.95 ± 0.23 1.42 ± 0.47 0.88 ± 0.42 1.42 ± 0.40 1.32 ± 0.45 1.86 ± 0.66 0.105 ± 0.013 100–149 6 121 ± 19 0.99 ± 0.28 0.81 ± 0.22 0.41 ± 0.15 0.72 ± 0.36 0.78 ± 0.21 0.99 ± 0.30 0.104 ± 0.009 50–99 3 74 ± 8 1.16 ± 0.65 0.57 ± 0.32 0.31 ± 0.09 0.55 ± 0.29 0.55 ± 0.32 0.67 ± 0.38 0.109 ± 0.006 0–49 5 31 ± 6 1.83 ± 0.91 0.22 ± 0.06 0.18 ± 0.05 0.22 ± 0.05 0.22 ± 0.06 0.22 ± 0.05 0.091 ± 0.023 Values are mean ± SD.
Jump to section
Related Articles
Cited By...
- Sympathetic Innervation of Cold-Activated Brown and White Fat in Lean Young Adults
- Mechanistic Insights into Sympathetic Neuronal Regeneration: Multitracer Molecular Imaging of Catecholamine Handling After Cardiac Transplantation
- Multiparametric Molecular Imaging Provides Mechanistic Insights into Sympathetic Innervation Impairment in the Viable Infarct Border Zone
- Presence of Specific 11C-meta-Hydroxyephedrine Retention in Heart, Lung, Pancreas, and Brown Adipose Tissue