OtherBASIC SCIENCE INVESTIGATIONS

Noninvasive Molecular Imaging to Detect Transgene Expression of Lentiviral Vector in Nonhuman Primates

William E. Sander, Mark E. Metzger, Kouki Morizono, Aylin Bonifacino, Scott R. Penzak, Yi-Ming Xie, Irvin S.Y. Chen, Jeffrey Bacon, Stephen G. Sestrich, Lawrence P. Szajek and Robert E. Donahue
Journal of Nuclear Medicine July 2006, 47 (7) 1212-1219;

Article Figures & Data

Figures

  • FIGURE 1. 
    FIGURE 1. 

    Flow cytometry data for monkey CJ2R. Top section contains dot plots for gated cell types: granulocytes, monocytes, lymphocytes, red blood cells, and CD41+ platelets and their respective percentages of prevalence of EGFP in blood at 14 mo. Bottom section contains bar graph of percentages of EGFP in different blood cell types over period of 1.5 y.

  • FIGURE 2. 
    FIGURE 2. 

    Flow cytometry data for monkey 2RC003. Top section contains dot plots for gated cell types: granulocytes, monocytes, lymphocytes, red blood cells, and CD41+ platelets and their respective percentages of prevalence of EGFP in blood at 14 mo. Bottom section contains bar graph of percentages of EGFP in different blood cell types over period of 1.5 y.

  • FIGURE 3. 
    FIGURE 3. 

    Two profiles of concentration vs. time showing distribution and elimination of 76Br-FBAU in monkeys CJ2R (A) and 2RC003 (B) at 3, 6, and 14 mo. Also shown is average radioactivity of whole blood for control monkeys, with 95% confidence bars, in becquerels per milliliter of blood taken at time points between 1 and 240 min.

  • FIGURE 4. 
    FIGURE 4. 

    Bar graph of average radioactive intensity for extremities as percentage of bladder radioactive intensity for control monkeys (n = 8). Monkeys CJ2R and 2RC003 were graphed in same way at 4 time points: 1 before and 3 after transplantation.

  • FIGURE 5. 
    FIGURE 5. 

    MedX (Medical Numerics)-prepared images of PET scans taken before transplantation (A and B) and 6 mo after transplantation (C and D) of hematopoietic stem cells transduced with lentiviral vector containing HSV1-tk for monkeys CJ2R (A and C) and 2RC003 (B and D), with image brightness, contrast, and intensity standardized between the two.

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